Multifunctional surface plasma coupled emission fluorescence and Raman detector and detection method thereof

Abstract

The invention discloses a multifunctional surface plasma coupled emission fluorescence and Raman detector and a detection method thereof, relating to detection of fluorescent spectrum, Raman spectrum,fluorescent imaging and fluorescent microscopic imaging. The detector comprises an angle distinguishing module, an excited light path module, a fluorescent spectrum detection module, a Raman spectrumdetection module, an imaging detection module and a microscopic imaging detection module, wherein the modules are separately fixed on corresponding rotary tables through optical guide rails, and canrotate simultaneously or independently around a prism and a membrane sample on the prism, excitation signals of different angles and emission signals of different angles can be generated by rotating the modules, and a square pyramid mirror is used as a coupling element and combines with the detection modules so as to enable the detector to acquire surface enhanced fluorescence and Raman, spectrumand imaging information and so on of the same sample simultaneously without using a light splitting system. The detector has a simple structure, is low-cost, is easy to operate, and is easy to promote.

Description

technical field [0001] The invention relates to integrated functional detection of fluorescence and Raman, spectrum and imaging, and in particular to a detection device and detection method for surface plasmon coupled emission fluorescence spectroscopy, Raman spectroscopy, fluorescence imaging, and fluorescence microscopic imaging. Background technique [0002] Fluorescence analysis technology has the advantages of high sensitivity and method diversity, and its application in the fields of industry and agriculture, quality inspection and scientific research is increasing day by day. However, in practical applications, due to the complexity and particularity of samples, the sensitivity and selectivity of existing conventional fluorescence methods cannot meet the needs of all detections. In order to detect components in low concentrations or complex substrates, researchers are still working on the development of new fluorescence techniques and instruments to further improve th...

AI Technical Summary

Problems solved by technology

However, the above-mentioned dual-mode SPCE imaging detection device based on the prism configuration still has disadvantages: SPCE has angular orientation, and a hemispherical prism is used as a coupling element signal to form a ring with uniform intensity in space, but the imaging device only The signal is collected at a certain angle, so the utilization rate of the enhanced fluorescent signal is not sufficient; only imaging is obtained through the CCD, and spectral information is not obtained; and only fluorescence detection is not realized, Raman detection is not realized, and the collected signal is relatively single, which limits SPCE Promotion of analytical techniques

Images