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Particle counting method

A particle and counter technology, applied in individual particle analysis, particle and sedimentation analysis, measurement devices, etc., can solve problems such as inaccurate results, reduce counting errors, reduce errors in cell life and death discrimination, and avoid image features. effect of difference

Inactive Publication Date: 2018-10-09
JIANGSU JIM BIO SCI & TECH
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Problems solved by technology

[0008] The purpose of the present invention is to provide a method for particle counting to alleviate the technical problems in the prior art that the amount of detection samples is small and the results are inaccurate caused by layered suspension of cells or other biological particles

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Embodiment Construction

[0051] The technical solutions of the present invention will be clearly and completely described below in conjunction with the embodiments. Obviously, the described embodiments are part of the embodiments of the present invention, but not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0052] In the description of the present invention, it should be noted that if the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer" appear ", etc., the indicated orientation or positional relationship is based on the orientation or positional relationship shown in the drawings, which is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the referred device or element must have a specific orientat...

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Abstract

The invention provides a particle counting method and relates to the technical field of biological particle detection. The particle counting method provided by the invention has the benefits that by adopting a microfluidic chip detection technology based on a Coulter principle, an electric pulse signal generated when cells and / or microbial samples flow through a detection area on the microfluidicchip is analyzed to obtain the size and the quantity of particles; the adopted microfluidic chip mainly reduces the stratified suspension problem of particle samples through the chip structure design(reducing the micro-channel depth) and effectively reduces the counting error and the cell death and life discrimination error caused by particle stratification during image acquisition in combinationwith the field depth of an optical system; the field depth of an optical lens is greater than the depth of the chip, so that the image feature difference caused by the stratified suspension is avoided; in addition, through the design of the chip, all the added quantitative samples can also flow through the detection area, so that the counting of all the samples is realized; in conclusion, the accuracy and the consistency of count results of the cells or other biological particles are much better than those obtained by an existing detection method.

Description

technical field [0001] The invention relates to the technical field of biological particle detection, in particular to a particle counting method. Background technique [0002] In a large number of cell biology research experiments, it is necessary to perform concentration detection (quantitative counting) on ​​cells or other biological particles. The concentration (or quantity) of cell suspension is not only a monitoring parameter for cell culture, but also a necessary parameter in many experimental projects. Completing the experiment is a very basic but important process condition. Existing counting methods mainly include manual counting methods using cell counting plates, automatic counting instruments based on image analysis technology, and automatic counting instruments using the resistance method (Coulter's principle). [0003] Among them, the manual counting method is the most common. The experimenter injects the suspended cell sample into the counting pool of the ce...

Claims

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Application Information

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IPC IPC(8): G01N15/12
CPCG01N15/12
Inventor 吴旭东帅宇董健张羽曹淙涵李会娟
Owner JIANGSU JIM BIO SCI & TECH
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