Characterization of mir-9 overexpressing tumors and their treatment and specific markers

A marker and characterization technology, applied in the field of pharmacy, can solve problems such as unclear role of exosomes

Inactive Publication Date: 2021-05-18
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although there is evidence that exosomes regulate tumor development and metastasis, the role of exosomes in tumor angiogenesis (especially after AATs) is unclear

Method used

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  • Characterization of mir-9 overexpressing tumors and their treatment and specific markers
  • Characterization of mir-9 overexpressing tumors and their treatment and specific markers
  • Characterization of mir-9 overexpressing tumors and their treatment and specific markers

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Experimental program
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Embodiment Construction

[0168] The present invention will be further illustrated by the following embodiments in conjunction with the accompanying drawings in order to provide a basis for better understanding of the present invention.

[0169] 1. Method

[0170] The serial numbers before the subheadings are for ease of reading and are for reference only and do not determine the order.

[0171] 1.1 Cell culture

[0172] HUVECs (Allcells, Shanghai, China) were cultured using HUVEC medium (HUVEC-004; Allcells). We use third to sixth generation HUVECs. The human HCC cell lines SMMC-7721 and Huh7 were purchased from the American Type Culture Collection (ATCC, Manassas, Virginia, USA) and cultured in Dulbecco's Modified Eagle's Nest with 10% fetal bovine serum (FBS; Hyclone). Growth was carried out in Hyclone medium high glucose medium (Hyclone; GE Healthcare, Logan, Utah, USA). Cells were maintained at 5% CO 2 , 37°C.

[0173] The MiR-9 mimic (5'-TCTTTGGTTATCTAGCTGTATGA-3') was inserted into the LV3...

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Abstract

The present invention provides a method for constructing tumors with high miR-9 expression, and found that vandetanib and 3-methyladenine can inhibit the tumor grafts constructed by this method, but lead to a large amount of VEGF-rich exosomes secretion. The present invention further provides corresponding treatment methods and therapeutic drugs or compositions of vandetanib and 3-methyladenine, a VEGF-rich exosome marker for evaluating the corresponding treatment process, and a method for characterizing the marker Reagent test kit. The present invention provides a new strategy for the treatment, prevention and therapeutic effect evaluation of tumors, especially tumors with high expression of miR-9.

Description

technical field [0001] The present invention relates to the field of pharmacy, relates to miR-9 high expression tumors and their treatment and characterization of specific markers, in particular to VEGF-rich exosomes in response to anti-angiogenesis and anti-autophagy therapy and their corresponding characterization and application. Background technique [0002] Anti-angiogenic therapies (AATs) aimed at treating tumors by inhibiting tumor angiogenesis and destroying existing tumor blood vessels have not achieved the desired effect 1,2 . The efficacy of AATs is short-lived, and after cessation of treatment, tumors relapse rapidly through different forms of angiogenesis, such as vessel selection, endothelial angiogenesis, and angiogenesis mimicry (VM). 3-7 . In addition, AATs can destroy the tumor vascular barrier, promote tumor cell invasion and metastasis, make cancer cells such as hepatocellular carcinoma (HCC) cells escape AATs, or form AATs resistance 6,7 . Metastasi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/867C12N5/10C12Q1/6886G01N33/569A61K31/517A61K31/52A61P35/00
CPCA61K31/517A61K31/52A61P35/00C12N15/86C12N2740/15043C12Q1/6886C12Q2600/178G01N33/56966A61K2300/00
Inventor 曾烨刘肖珩
Owner SICHUAN UNIV
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