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Four astraisoflavan-7-O-beta-D-glucoside compounds with nerve cell protection activity and preparation method thereof

A nerve cell protection and isoflavane glycoside technology, which is applied in the field of four isoflavane glycoside compounds and their preparation, can solve the problems of unseen compounds

Active Publication Date: 2018-10-02
SHENYANG INSTITUTE OF CHEMICAL TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

After investigation, there is no relevant report on the compound of the present invention and its activity of anti-neurodegenerative disease value therapeutic prospect in the prior art

Method used

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  • Four astraisoflavan-7-O-beta-D-glucoside compounds with nerve cell protection activity and preparation method thereof
  • Four astraisoflavan-7-O-beta-D-glucoside compounds with nerve cell protection activity and preparation method thereof
  • Four astraisoflavan-7-O-beta-D-glucoside compounds with nerve cell protection activity and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Isolation and purification of compound 1-4:

[0047] Medicago sativa ( Medicago sativa L.) roots 7 kg, 70% ethanol was heated and refluxed for extraction three times, each for 2 hours. The extract was concentrated under reduced pressure to obtain the total extract. The total extract was suspended and dispersed in water, extracted three times with dichloromethane, and concentrated under reduced pressure to obtain 1.5 kg of aqueous layer extract. Separate 1.5 kg of the aqueous layer extract with macroporous resin D101, perform gradient elution according to methanol:water 0:100, 30:70, 50:50, 70:30, 100:0, and concentrate under reduced pressure to obtain fraction Fr . AE five stream points. The component Fr. D was subjected to normal phase silica gel column chromatography, and the ratio of dichloromethane: methanol was 50:1, 30:1, 15:1, 8:1, 4:1, 2:1, 1:1, 0:1 gradient elution was carried out, and 8 fractions (Fr. D1-D8) were obtained, of which D3 was subjected to silica g...

Embodiment 2

[0060] The pharmacological effects of the compound of the present invention and the pharmaceutical composition composed of pharmaceutical excipients in neurodegenerative diseases.

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Abstract

The invention discloses four astraisoflavan-7-O-beta-D-glucoside compounds with nerve cell protection activity and a preparation method thereof, and relates to a pharmaceutical compound and a preparation method thereof. Four novel astraisoflavan-7-O-beta-D-glucoside compounds are separated from alfalfa roots. Four novel astraisoflavan-7-O-beta-D-glucoside compounds are separated from alfalfa rootsby an activity tracking technology and then purified. Four novel compounds can prevent the H2O2 induced SH-SY5Y nerve cell damage and well protects the nerve cells. An H2O2 induced SH-SY5Y nerve celldamage model is used to evaluate the nerve cell protection performance of the compounds; a prominent dose-effect relationship is represented, and thus the compounds have a good application prospect in the preparation of drugs for treating neurodegenerative diseases.

Description

Technical field [0001] The invention relates to a pharmaceutical compound, in particular to a four isoflavone glycoside compound with neuroprotective activity and a preparation method thereof. Background technique [0002] With the increasingly prominent problem of population aging, neurodegenerative diseases have become one of the most serious diseases that endanger human health in the world. Neurodegenerative diseases are a type of neurological diseases characterized by the progressive damage of specific neurons, including Alzheimer disease (AD), Parkinson disease (PD), Huntington disease (Huntington disease) disease, HD) and amyotrophic lateral sclerosis. The pathogenesis of neurodegenerative diseases is extremely complex and has not been fully elucidated so far. There is a lack of effective diagnosis and treatment methods. A large number of studies have shown that oxygen free radical damage (Oxidative Stress) plays an important role in the mechanism of neurodegenerative dis...

Claims

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Application Information

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IPC IPC(8): C07H15/26C07H17/065C07H17/04C07H1/08A61K31/7048A61P25/28A61P25/00
CPCA61P25/00A61P25/28C07H1/08C07H15/26C07H17/04C07H17/065
Inventor 刘学贵张立新李丹琦高品一
Owner SHENYANG INSTITUTE OF CHEMICAL TECHNOLOGY
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