Visual nucleic acid detection method for canine distemper viruses
A technology of canine distemper virus and detection method, which is applied in the field of visual and rapid detection of canine distemper virus, can solve the problems of cumbersome operation and low specificity, and achieve the effect of solving cumbersome operation
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[0030] A method for detecting canine distemper virus
[0031] 1. Materials and methods
[0032] 1.1 Virus strains
[0033] Canine distemper virus (vaccine strain Onderstepoort), canine parvovirus (CPV), canine adenovirus (CAV), canine parainfluenza virus (CPIV), canine distemper 10th generation strain; Vero cells; CDV plasmids all provided by military veterinarians Stored in the virus room of the research institute;
[0034] 1.2 Main reagents
[0035] Bst DNA polymerase, MgSO 4 , 10×Amplification buffer, and AMV enzyme were purchased from NEB Company; dNTP was purchased from Solarbio Company; betaine was purchased from Sigma Company; viral genome DNA / RNA extraction kit was purchased from Tiangen Biochemical Technology (Beijing) Co., Ltd.; disposable nucleic acid The detection device (No. 3) was purchased from Hangzhou Ustar Biotechnology Co., Ltd.;
[0036] 1.3 Primer design
[0037] According to the CDV gene sequence included in GenBank, DNAM software was used for compa...
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