A Molecular Marker Related to Cotton Fiber Length and Its Application
A fiber length and upland cotton technology, applied in the field of molecular markers, can solve the problems of slow progress in fiber length breeding, poor accuracy and low efficiency, and achieve the effects of speeding up the process of cultivation and seed industrialization, solving slow progress and overcoming high costs.
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Embodiment 1
[0047] Example 1: Screening molecular markers
[0048] (1) Group construction and data acquisition
[0049] Using Haidao Mianhai 1 as the donor parent and Zhongmiansuo 45 (Zhong 45) as the recurrent parent, cross backcross and self-cross to obtain Zhongmiansuo 45×Hai 1BC 4 f 3:5 Chromosomal segment substitution lines were evaluated in multiple environments (Ma Liujun et al., 2013), and a high-quality substitution line MIBI7749 containing a small number of introgression segments was selected as the female parent and crossed with the reincarnated parent Zhongmiansuo 45 as the male parent. Constructing a secondary segregation large population F 2 .
[0050] Planting F in Anyang in 2013 2 A large group of 900 plants was isolated, with a row length of 8m, a row width of 80cm, and a plant spacing of 25cm. The individual plants were subjected to routine field investigation and fiber quality determination, and 604 individual plants were measured for fiber length. Among them, 82 a...
Embodiment 2
[0072] Example 2: Molecular marker selection method for improving fiber length traits of upland cotton
[0073] Using the molecular marker CGR6381 obtained in Example 1 150 、HAU0734 210 and CGR5151 140 Molecular marker selection is carried out in the breeding population related to Sea Island Cotton Sea 1, etc., including the following steps:
[0074] (1) DNA extraction: take Haidao Mianhai 1 as the donor parent, and upland cotton varieties or strains (such as Zhongmian Institute 60, Lumianyan 28, Xinluzao 50, Ji 08) as the recipient parent, carry out hybridization, return Cross to obtain segregated populations, or use Haidao Mianhai 1 as the donor parent and upland cotton varieties (such as Zhongmian 60, Lumianyan 28, Xinluzao 50, Ji 08) as recipient parents to obtain high-generation backcross The substituent line and its derivatives, or the progeny of the hybridization and backcrossing of the substitution line MBI7747 and the upland cotton variety, the DNA of a single plan...
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