A Molecular Marker Related to Cotton Fiber Length and Its Application

A fiber length and upland cotton technology, applied in the field of molecular markers, can solve the problems of slow progress in fiber length breeding, poor accuracy and low efficiency, and achieve the effects of speeding up the process of cultivation and seed industrialization, solving slow progress and overcoming high costs.

Active Publication Date: 2021-11-23
INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to overcome many shortcomings such as poor accuracy of phenotypic selection, low efficiency, long cycle and high cost in traditional breeding, and solve the problem of slow progress in fiber length breeding

Method used

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  • A Molecular Marker Related to Cotton Fiber Length and Its Application
  • A Molecular Marker Related to Cotton Fiber Length and Its Application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Example 1: Screening molecular markers

[0048] (1) Group construction and data acquisition

[0049] Using Haidao Mianhai 1 as the donor parent and Zhongmiansuo 45 (Zhong 45) as the recurrent parent, cross backcross and self-cross to obtain Zhongmiansuo 45×Hai 1BC 4 f 3：5 Chromosomal segment substitution lines were evaluated in multiple environments (Ma Liujun et al., 2013), and a high-quality substitution line MIBI7749 containing a small number of introgression segments was selected as the female parent and crossed with the reincarnated parent Zhongmiansuo 45 as the male parent. Constructing a secondary segregation large population F 2 .

[0050] Planting F in Anyang in 2013 2 A large group of 900 plants was isolated, with a row length of 8m, a row width of 80cm, and a plant spacing of 25cm. The individual plants were subjected to routine field investigation and fiber quality determination, and 604 individual plants were measured for fiber length. Among them, 82 a...

Embodiment 2

[0072] Example 2: Molecular marker selection method for improving fiber length traits of upland cotton

[0073] Using the molecular marker CGR6381 obtained in Example 1 150 、HAU0734 210 and CGR5151 140 Molecular marker selection is carried out in the breeding population related to Sea Island Cotton Sea 1, etc., including the following steps:

[0074] (1) DNA extraction: take Haidao Mianhai 1 as the donor parent, and upland cotton varieties or strains (such as Zhongmian Institute 60, Lumianyan 28, Xinluzao 50, Ji 08) as the recipient parent, carry out hybridization, return Cross to obtain segregated populations, or use Haidao Mianhai 1 as the donor parent and upland cotton varieties (such as Zhongmian 60, Lumianyan 28, Xinluzao 50, Ji 08) as recipient parents to obtain high-generation backcross The substituent line and its derivatives, or the progeny of the hybridization and backcrossing of the substitution line MBI7747 and the upland cotton variety, the DNA of a single plan...

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Abstract

The invention relates to the technical field of molecular breeding, in particular to a molecular marker related to fiber length from Sea Island Cotton Sea 1 and its application. The molecular marker is CGR6381 150 、HAU0734 210 and CGR5151 140 . The invention helps to overcome the deficiency of fiber length identification in the existing breeding technology, improves the selection efficiency of fiber length, and accelerates the cultivation process of high-quality new varieties.

Description

technical field [0001] The invention relates to the technical field of cotton molecular breeding, in particular to a molecular marker related to fiber length from Sea Island Cotton Sea 1 and its application. Background technique [0002] Cotton is an important economic crop in the world, and cotton fiber is an important raw material for textile industry. Cotton occupies an important position in my country's national economy. With the rapid development of the textile industry and the continuous improvement of people's living standards, the requirements for the quality of cotton fibers are also getting higher and higher. Upland cotton varieties are planted in a large area in my country. Before the 1980s, cotton breeders mainly focused on improving the yield, but did not pay enough attention to the improvement of fiber quality. As a result, most of the cotton varieties in my country showed high yield and high quality. Difference. In the middle and late 1990s, fiber quality bre...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13
Inventor 卢全伟石玉真袁友禄肖向辉刘爱英龚举武李俊文葛群巩万奎商海红潘境涛
Owner INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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