Thyroid stimulating hormone TSH kit based on microfluidic chip and preparation and detection methods

Abstract

The invention discloses a thyroid stimulating hormone TSH kit based on a microfluidic chip and preparation and detection methods. The preparation method of the thyroid stimulating hormone TSH kit comprises the following steps: (1) coating the microfluidic chip; (2) labeling fluorescent microspheres; (3) assembling the microfluidic chip; (4) preparing a scaling product; (5) obtaining the thyroid stimulating hormone TSH kit based on the microfluidic chip. The prepared thyroid stimulating hormone TSH kit adopts a double-antibody sandwich method to carry out measurement, selects a time-resolutionfluorescent substance with high sensitivity as a labeling substance, carries out antibody labeling on the fluorescent microspheres and utilizes immunoreaction of antibody pairs to carry out analysis and detection, and the property of the prepared reagent can reach the level of an equivalent chemiluminescence reagent. After immunoreaction, cleaning solution is used for completely removing redundantcomponents, detection reading is carried out on the fluorescent microspheres combined by the immunoreaction, so that the problem that developing solution is introduced into the chip to cause incomplete reaction or untimely reading after developing is avoided.

Description

technical field [0001] The invention belongs to the field of immune detection, and in particular relates to a microfluidic chip-based thyroid-stimulating hormone TSH detection kit and a preparation and detection method thereof. Background technique [0002] Microfluidic chip technology is characterized by a micropipeline network, using micromachining technology to etch a micropipeline network and other functional units on a chip of a few square centimeters in size, thereby preparing a microfluidic chip that includes injection, reaction, separation, and detection An integrated fast, efficient, and low-consumption micro-analysis device. The microfluidic chip has the characteristics of less reagent consumption, short reaction time and high degree of automation in the detection platform. In the current research, the miniaturization of the chip has brought many difficulties to the early sample separation and supporting integration, and there are also many problems in sample fixa...

AI Technical Summary

Problems solved by technology

[0014] Using other existing technologies or (1) the structure of the chip is too complicated to precisely regulate the reaction, and there is a large pollution in the cleaning steps and liquid conduction; or (2) multiple liquid chambers and solid phase carriers are used (magnetic beads and fluorescent microspheres are used at the same time), the overall design of the reagent is too complicated; or (3) the immunoreaction adopts a heterogeneous reaction, which means that the solid phase carrier reacts with the surface of the solid phase carrier, the reaction is insufficient, and the sensitivity and Repeatability is limited

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