A line confocal imaging device with multi-channel scanning and detection

Abstract

The invention discloses a wire confocal imaging device for multichannel scanning and detecting, which comprises an exciting light module, a scanning imaging module and a detecting module. The excitinglight module is used for providing two separated linear beam patterns obtained through shaping two laser beams; the inclination angles and the divergence angles of the laser beams are adjustable; thescanning imaging module is used for focusing the two linear beam patterns to different imaging positions, so that two imaging light beams are produced, and three-dimensional scanning imaging is realized; the detecting module is used for separating and detecting the two imaging light beams; through fine adjusting the inclination angles and the divergence angles of the two laser beams in the exciting light module, simultaneous scanning image of double focal planes or simultaneous scanning imaging of double lines in the same focal plane of a simple objective angular field can be realized. According to the wire confocal imaging device for multichannel scanning and detecting provided by the invention, the imaging speed can be effectively improved, and meanwhile, the system adjustment can be carried out conveniently.

Description

technical field [0001] The invention belongs to the field of microscopic imaging, and more specifically relates to a line confocal imaging device for multi-channel scanning and detection. Background technique [0002] Microscopic imaging technology is widely used in the fields of life science and medicine. Imaging speed is an important parameter in the field of microscopic imaging. Especially for the imaging of large volume samples, it is particularly important to obtain image information in a short time. According to the shape of excitation light, microscopic imaging methods can be divided into point scanning, line scanning and area scanning. The most common application of point scanning is laser scanning confocal microscopy. This imaging method places a small hole in front of the detector to filter out the interference of non-focal plane information, forming an optical tomography, thereby improving image contrast; confocal microscopy scans the sample point by point In ord...

AI Technical Summary

Problems solved by technology

However, if you want to image a large-volume sample at the centimeter level, you need to introduce a three-dimensional mobile stage to drive the sample to perform mosaic scanning and imaging. Since the system does not image when the platform moves from the current position to the next imaging position, the larger the sample , the more times the platform moves, the more non-imaging time, which affects the imaging speed and imaging efficiency

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