Molecular marker breeding method for improving natural immunity of pigs and application of molecular marker breeding method

Abstract

The invention provides a molecular marker breeding method for improving the natural immunity of pigs and application of the molecular marker breeding method, relates to the field of molecular geneticsand in particular relates to application of a molecular marker method of a mutation site of an Sp100 gene 5' regulation region of the pigs in disease-resisting breeding of the pigs. The inventor finds out that the Sp100 gene 5' regulation regions of Hebao pigs and large and white pigs have many differences, wherein a -283 site has mutation from C to G; a luciferase reporter gene system finds outthat after the C of the -283 site is changed into the G through site-directed mutation, the activity of a promoter is remarkably improved; the mRNA (messenger Ribonucleic Acid) expression amount is low and is the same as that of the Hebao pigs with strong disease resistance. Visibly, a gene type of the -283 site of the Sp100 regulation region in a genome of the pig can be detected and used as a molecular marker related to immune performance of the pigs; the method is simple and rapid and is not influenced by the environment, and early-period seed selection can be realized.

Description

technical field [0001] The present invention relates to the field of molecular genetics, in particular to a breeding method for improving the natural immunity of pigs. The polymorphism of the mutation site in the 5' regulatory region of the pig Sp100 gene is selected for selection, and the mutation site is artificially applied. Point to achieve high disease resistance pig breeding. Background technique [0002] my country is the largest pork production and consumption country in the world. Pork is the main meat of our people, accounting for 50-60% of the total meat consumption. Improving economic traits such as growth rate, lean meat percentage, meat quality and litter size in pigs has always been a priority for pig producers and pig breeders. Over the past few decades, important economic traits such as daily gain, carcass lean percentage, backfat thickness, and feed remuneration of pigs have improved significantly. However, because there is a certain degree of negative co...

AI Technical Summary

Problems solved by technology

However, due to the complex genetic basis of disease resistance traits, the difficulty of determining disease resistance or susceptibility indicators, the difficulty of measuring offspring, and the long generation interval, conventional breeding is difficult to achieve ideal results.

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