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Chitosan Compound bacterial agent as well as preparation method and application thereof

A compound bacterial agent and chitosan technology, applied in the direction of botany equipment and methods, applications, fungicides, etc., can solve the problems of poor inhibition effect, achieve the effects of reducing the incidence rate, promoting growth, and promoting the decomposition of organic matter

Inactive Publication Date: 2018-07-24
厦门昶青生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of this, in order to solve the problem of poor inhibition of bacteria and fungi at higher concentrations of chitosan, the invention provides a chitosan composite bacterial agent

Method used

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Examples

Experimental program
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Effect test

preparation example Construction

[0062] The present invention also provides the preparation method of chitosan composite bacterial agent described in above-mentioned technical scheme, comprises the following steps:

[0063] S1, mixing chitosan and Hoagland's nutrient solution according to the mass volume ratio to obtain a chitosan solution;

[0064] S2. Mix the chitosan solution and the bacillus solution according to the volume ratio of the used Hoagland's nutrient solution and the bacillus solution to obtain a chitosan composite bacterial agent.

[0065] In the present invention, there is no special limitation on the mixing conditions, and conventional mixing methods can be used.

[0066] The chitosan composite bacterial agent of the present invention can also be used in plant protection, specifically, the chitosan composite bacterial agent of the present invention can be used in promoting plant growth and preventing and controlling plant microbial diseases, and the shell The polysaccharide compound bacteri...

Embodiment 1

[0071] Washing the shrimp shells of Penaeus vannamei into clean shells, drying the washed shells of Penaeus vannamei with a water content of 0.1% at 70° C., to obtain dried shells of Penaeus vannamei. Mix 100g of Penaeus vannamei shrimp shells with 2L of absolute ethanol, react at 50°C for 3 hours for crude extraction, filter after the reaction, and the solid part is the primary material.

[0072] The obtained primary material and neutral protease solution were mixed according to the ratio of material to liquid 1:2, enzymolyzed at 45°C and pH 7.0 for 4 hours, filtered to obtain enzymolyzed protein solution and shrimp shell enzymolyzed residue, Enzymatic protein solution can be used as raw material for protein processing. Wherein, the enzyme content in the neutral protease solution is 1000U / g.

[0073]Soak the obtained shrimp shell enzymatic hydrolysis residue in 6% hydrochloric acid solution for 4 hours at a soaking temperature of 30° C., and filter to obtain crude chitin. T...

Embodiment 2

[0076] Washing the shrimp shells of Penaeus vannamei into clean shells, drying the washed shells of Penaeus vannamei with a water content of 0.5% at 65° C., to obtain dried shells of Penaeus vannamei. Mix 100g of Penaeus vannamei shrimp shells with 1.5L of absolute ethanol, react at 50°C for 6h for crude extraction, filter after the reaction, and the solid part is the primary material.

[0077] The obtained primary material and neutral protease solution were mixed according to the ratio of material to liquid 1:1.5, enzymolyzed at 42°C and pH6.8 for 6 hours, filtered to obtain enzymolyzed protein solution and shrimp shell enzymolyzed residue, Enzymatic protein solution can be used as raw material for protein processing. Wherein, the enzyme content in the neutral protease solution is 800U / g.

[0078] Soak the obtained shrimp shell enzymatic hydrolysis residue in 4% hydrochloric acid solution for 6 hours at a soaking temperature of 28° C., and filter to obtain crude chitin. The...

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Abstract

The invention relates to the field of plant protective preparations and provides a chitosan compound bacterial agent. The compound bacterial agent is prepared from the following components of: chitosan, bacillus solution and Hoagland's nutrient solution, wherein the mass-volume ratio of chitosan and the Hoagland's nutrient solution is 10-25g:90-120mL, and the volume ratio of the Hoagland's nutrient solution and bacillus subtilis is (8-10):1. The chitosan compound preparation adopts chitosan and the bacillus solution as main active substances, and by complexing of chitosan and the bacillus solution, the antibacterial effect of chitosan for aspergillus niger under a higher concentration can be improved. The chitosan compound bacterial agent also can be used in plant protection, and also hasthe effects of adjusting the structure of microorganisms in root-system soil of plants, promoting decomposition of organic matters and generating rich metabolites while playing an antibacterial role so as to play roles in promoting the growth of the plants and protecting the plants.

Description

technical field [0001] The invention relates to the field of plant protection preparations, in particular to a chitosan composite bacterial agent and a preparation method thereof, as well as the application of the chitosan composite bacterial agent in plant protection. Background technique [0002] Chitosan (chitosan), also known as deacetylated chitin, the chemical name is polyglucosamine (1-4)-2-amino-B-D glucose, is the product of chitin after deacetylation treatment, is the most basic chitin , the most important derivative lysate. Chitosan is widely used because of its non-toxic side effects, microbial degradation, good biocompatibility and film-forming properties. Chitosan is easy to dissolve in a weak acid solvent, and it is particularly worth noting that the dissolved solution contains amino groups (-NH 2 ), these amino groups inhibit bacteria by binding negative electrons. The antibacterial activity of chitosan makes it widely used in the fields of medicine, texti...

Claims

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Application Information

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IPC IPC(8): A01N63/00A01N43/16A01P1/00A01P3/00A01P21/00C05G3/00
CPCA01N43/16A01N63/00C05F11/00C05G3/00C05G5/20
Inventor 姜宗然林茂吴少坤郭立
Owner 厦门昶青生物科技有限公司
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