Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Methods for generating engineered human primary blood dendritic cell lines

A technology of dendritic cells and cells, applied in the fields of biology and medicine

Pending Publication Date: 2018-07-17
UNIV OF MARYLAND BALTIMORE
View PDF10 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, HTLV-2 is related to its pathogenic counterpart HTLV-1, however, HTLV-2 has not been linked to the development of leukemia in humans

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Methods for generating engineered human primary blood dendritic cell lines
  • Methods for generating engineered human primary blood dendritic cell lines
  • Methods for generating engineered human primary blood dendritic cell lines

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0251] The dendritic cells described herein and their preparation methods are particularly useful in the preparation of vaccines. Accordingly, provided herein are related dendritic cell compositions and vaccines. In one embodiment, the vaccine is autologous to the subject. In some embodiments, the vaccine is allogeneic to the subject. In some embodiments, the dendritic cell vaccine is loaded with antigens from cancer cells or pathogens present in the subject.

[0252] Mature or immature, antigen-loaded or non-antigen-loaded dendritic cells can be frozen in a composition comprising a cryoprotectant. Numerous cryoprotectants are known to those skilled in the art. Examples of cryoprotectants include, but are not limited to, dimethyl sulfoxide (DMSO), glycerol, ethanol, methanol, acetamide, glycerol monoacetate, propane-diol, polyethylene glycol, ethylene glycol, isoerythrose alcohol, D-ribitol, D-mannitol, D-sorbitol, D-lactose, isoinositol, choline chloride, amino acid, albu...

Embodiment 1

[0483] Example 1. Generation of Human Primary Blood and Monocyte-Derived Dendritic Cell Lines

[0484] HTLV-2 Tax has been used as a molecular tool due to its role in modulating DC function. To prevent potential loss of DCs during culture of Tax-transduced PBMCs, transduced cells were negatively selected using anti-CD3 magnetic beads to deplete T cells. Two CD3-negative Tax-GFP+ cell lines from transduced PBMCs from 10 blood donors evolved and grew continuously over 6 months without loss of growth potential. These two DC cell lines established from 2 different blood donors showed dendritic cell-associated markers. These cells were lineage-negative and expressed CD11c and CD205, as well as DC maturation and activation molecules, including CD83, CD80, CD86, CD70, CCR7, and HLA-DR (Figures 1a and 1b). These two cell lines, named ihv-DC1 and ihv-DC2, represent a subset of human CD11c+ / CD205+ blood dendritic cells that maintain a constitutively mature and activated phenotype.

...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention provides an engineered primary blood and monocyte-derived dendritic cells, wherein the dendritic cell expresses a functional Tax protein from a T cell leukemia virus, and methodsof making and using the same.

Description

[0001] Cross References to Related Applications [0002] This application claims the benefit of US Provisional Application No. 62 / 243,177, filed October 19, 2015, the contents of which are hereby incorporated by reference in their entirety. [0003] Incorporation of References for Electronic Submissions [0004] Incorporated herein in its entirety by reference is the computer readable sequence listing, filed concurrently with this document and identified as follows: A 60,990 byte ASCII (text) file named "sequence_listing_ST25.txt" created on October 19, 2016 document. field of invention [0005] The field of the invention relates generally to the fields of biology and medicine. The field also relates to methods for generating dendritic cells and related compositions useful in the treatment of diseases such as cancer. Background of the invention [0006] Among professional antigen-presenting cells (APCs), dendritic cells (DCs) have a unique ability to sensitize naive T lym...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/15A61K39/395C07K14/54C07K16/24A61P35/02A61P31/14C12N5/0784C12N15/62C12N15/86
CPCA61K39/395A61K35/15C07K14/005C12N2740/14022C12N2740/16043C07K14/4703C12N9/1276C07K2319/00C12N2510/00A61P35/02C12N5/0639A61K39/0011C07K14/15C12N15/86
Inventor H·程
Owner UNIV OF MARYLAND BALTIMORE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com