Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A tissue culture method for Rhododendron jinggangshanicum Tam

A technique of tissue culture and rhododendron, applied in the field of plant propagation, can solve the problems of long breeding time and low seed germination rate

Inactive Publication Date: 2018-06-29
JINGGANGSHAN UNIVERSITY
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Jinggangshan Rhododendron mainly reproduces by seeds, but the seed germination rate is low and the breeding time is long, so an effective rapid propagation method is still needed to propagate and protect it

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A tissue culture method for Rhododendron jinggangshanicum Tam
  • A tissue culture method for Rhododendron jinggangshanicum Tam
  • A tissue culture method for Rhododendron jinggangshanicum Tam

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0007] The adventitious buds are taken from the top buds or side buds of wasabi, washed with tap water, peeled off the outermost layer under aseptic conditions, and then sterilized with 70% alcohol for 30-60 s, 0.1% mercuric chloride for 10 minutes, Inoculated in adventitious bud induction medium (Read+NAA0.5 mg·L -1 +6-BA 2.0mg·L -1 + sucrose 20 g·L -1 + agar 7 g·L -1 ,pH 5.6), cultured at 25±2°C for 40 days, inoculated every 35 days in the subculture medium (Read + 6-BA 1 mg L -1 +ZT0.5mg·L -1 + sucrose 20 g·L -1 + agar 7 g·L -1 , pH5.6. When the sprouts grow to about 2cm, they are cut into single shoots and inoculated into the rooting medium (Read + IBA 1mg L -1 +BA0.1mg·L -1 + 30 g·L sucrose -1 + agar 7 g·L -1 , pH 5.6) for 40 days, loosen the bottle cap of the culture bottle, train the seedlings in the acclimatization room for 3 days, then take out the tissue culture seedlings from the bottle, clean the remaining medium for acclimation culture. The culture subs...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

A tissue culture method for Rhododendron jinggangshanicum Tam is disclosed. The method is characterized in that lateral buds and terminal buds of the Rhododendron jinggangshanicum Tam are induced through tissue culture to obtain primary sterile test-tube plantlets, and after subculture and rooting culture, the sterile seedlings are acclimatized to obtain tissue culture seedlings of the Rhododendron jinggangshanicum Tam. Through the method, a large number of rooted seedlings can be obtained in a short period, and production is not limited by seasons. The method provides a novel route for propagation and protection of the Rhododendron jinggangshanicum Tam that is an endemic species in China, and is of great significance.

Description

technical field [0001] The invention relates to a method for plant propagation, and mainly relates to a method for tissue culture of rhododendron in Jinggangshan. Background technique [0002] Jinggangshan Rhododendron (Rhododendron jinggangshanicum Tam) is an evergreen woody plant of Rhododendronaceae Rhododendron. Beautiful, bright colors, with high garden ornamental value. Rhododendron Jinggangshan mainly reproduces by seeds, but the seed germination rate is low and the breeding time is long, so an effective rapid propagation method is still needed for its propagation and protection. Tissue culture technology is a rapid breeding method emerging in the contemporary era. It is not only simple, rapid, and fast in reproduction, but also conducive to the promotion, large-scale production and protection of new plant varieties. Contents of the invention [0003] The object of the present invention is to establish a method for tissue culture of Rhododendron Jinggangshan to pr...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 曾建军邱洪龙
Owner JINGGANGSHAN UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com