Detection and application of a SNP site and molecular marker associated with resistance or susceptibility to diarrhea in suckling piglets
A locus, piglet technique, applied in the field of molecular genetics
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specific Embodiment approach 1
[0025] Specific embodiment one: A kind of SNP site related to suckling piglet diarrhea according to this embodiment, the SNP site is NC_010457.5:g.107288753C>A, located at 1063bp of the CTLA4 gene sequence, the CTLA4 gene sequence is as follows Shown in SEQ ID NO.1.
specific Embodiment approach 2
[0026] Embodiment 2: The molecular marker detection technology based on the SNP site described in Embodiment 1, the molecular marker detection technology is the PstI PCR-RFLP method.
specific Embodiment approach 3
[0027] Specific embodiment three: the sequence of the primer based on the PstI PCR-RFLP detection technology of the SNP site described in specific embodiment two is as follows:
[0028] C3F: 5'AGGTGCTTTCTCCTATTT 3'
[0029] C3R: 5'TTCTGTCTGTGGCATTAA 3'.
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