Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A Yeast Strain for Degrading Petroleum Aromatic Hydrocarbons and Its Application

An aromatic hydrocarbon and yeast technology, applied in the field of microbial degradation of petroleum pollutants, can solve problems such as differences in crude oil degradation capacity, and achieve the effects of high efficiency and rapid degradation capacity, good application prospects and economic value

Active Publication Date: 2021-09-17
YANGTZE UNIVERSITY
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Microbial degradation has been proven to be a very effective oil pollution removal technology, which has the advantages of cost-effectiveness, environmental friendliness, and no secondary pollution. The key to this technology is to screen out microorganisms that can efficiently degrade target pollutants. Due to the poor tolerance of degrading microorganisms to the growth environment and degradation environment, there is a large difference in the degradation ability of crude oil. Degradation characteristics, providing strains and genetic materials for biological remediation of crude oil polluted environment

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A Yeast Strain for Degrading Petroleum Aromatic Hydrocarbons and Its Application
  • A Yeast Strain for Degrading Petroleum Aromatic Hydrocarbons and Its Application
  • A Yeast Strain for Degrading Petroleum Aromatic Hydrocarbons and Its Application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0027] The present invention screens and confirms the bacterial strain type through the following methods, and provides various excellent properties of the bacterial strain at the same time, and the specific content is as follows:

[0028] 1 Materials and methods

[0029] 1.1 Collection of oil-contaminated soil samples

[0030] The oil-contaminated soil samples came from the oil-contaminated soil in the oil production area of ​​Qianjiang Guanghua Oilfield.

[0031] The moisture content of the polluted soil was determined to be 35%, and the pH value was 8.0.

[0032] 1.2 Medium

[0033] 1.2.1 Inorganic salt medium

[0034] NaNO 3 1.5 g, (NH 4 ) 2 SO 4 1.5 g, K 2 HPO 4 1.0 g, MgSO 4 ·7H 2 O 0.5 g, KCl 0.5 g, NaCl 5.0 g, FeSO 4 ·7H 2 O 0.01 g, CaCl 2 0.02 g, 1000 mL distilled water, pH7.0, 0.5% petroleum, 100 mL per Erlenmeyer flask.

[0035] 1.2.2 Fungal selective medium (Martin-Bengal red medium)

[0036] Glucose 10 g, peptone 5 g, K2HPO4 1 g, MgSO4 7H2O 0.5...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
clearance rateaaaaaaaaaa
Login to View More

Abstract

The invention discloses a petroleum aromatic hydrocarbon degrading yeast G-94, which is named as Candida tropicalis in taxonomy Candida Tropicalis .G‑94, belonging to the genus Yeast, was deposited in the China Center for Type Culture Collection (CCTCC) on June 30, 2017. The deposit address is Wuhan University, China, and the deposit number is: CCTCC NO: M 2017402. The strain Obtained by screening from petroleum-contaminated soil in the oil production area of ​​Qianjiang Guanghua Oilfield, the bacterial strain G-94 is ellipsoidal, and the bacterial colony is milky white. The concentration is 0.3%, the salt concentration is 0.5%, the inoculation amount is 4%, the N source (NH 4 ) 2 SO 4 , P source K 2 HPO 4 The degradation rate of petroleum aromatic hydrocarbons reached 67.32% after 30 days of culture under the conditions of the above conditions, and it has good application prospects and economic value in eliminating petroleum pollutants and biologically remediating petroleum aromatic hydrocarbons polluted soil and polluted water bodies.

Description

technical field [0001] The invention relates to a yeast strain for degrading petroleum aromatic hydrocarbons and its application, belonging to the technical field of microbial degradation of petroleum pollutants. Background technique [0002] During the extraction, transportation, storage, refining and use of crude oil, a large amount of crude oil and its derivative products inevitably enter the environment, causing serious pollution to soil and water. The chemical composition of crude oil is complex, including various toxic substances such as alkanes, cycloalkanes, and aromatic hydrocarbons. Some of these aromatic compounds have potential or proven carcinogenic, mutagenic, and mutagenic effects, and can pass through the food chain in animals, plants, and humans. The accumulation in the body poses a huge threat to the ecological environment and human health. [0003] Microbial degradation has been proven to be a very effective oil pollution removal technology, which has the...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20B09C1/10C02F3/34C12R1/74C02F101/32
CPCB09C1/10C02F3/347C02F2101/32C12N1/165C12R2001/74
Inventor 马立安詹亚斌余维初陶兴玲何山文宋淑芬幸晶晶
Owner YANGTZE UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com