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A kind of arginine-rich spirulina culture method

A cultivation method and technology of spirulina, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems affecting the production of arginine in spirulina, difficult industrial production, and failure to achieve health care, etc., to achieve Improve the level of high value-added industrial chain, low training costs, and improve product output

Active Publication Date: 2021-03-02
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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Problems solved by technology

Spirulina cells contain arginine, but under normal culture conditions, the content of arginine in spirulina is very small, less than 3% of the dry weight of cells, which cannot achieve the function of health care, and also affects the production of arginine in spirulina , which increases the cost of subsequent processing and extraction, making it difficult for industrial production

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  • A kind of arginine-rich spirulina culture method
  • A kind of arginine-rich spirulina culture method

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Embodiment 1

[0021] To investigate the accumulation of arginine in Spirulina under sulfur-limited conditions.

[0022] The algae cultivation and arginine accumulation processes were carried out in a 500mL cylindrical bubbling photobioreactor (glass material, diameter 50mm, height 400mm).

[0023] 1) Cultivation of algal species

[0024] Spirulina cells are cultured in a nutrient-rich medium (Zarrouk) prepared with deionized water containing: NaHCO per liter of water 3 16.8g, K 2 HPO 4 0.5g, NaNO 3 2.5g, K 2 SO 41g, NaCl 1g, MgSO 4 ·7H 2 O 0.2g, CaCl 2 2H 2 O 0.04g, FeSO 4 ·7H 2 O 0.01g, H 3 BO 3 2.86mg, MnCl 2 4H 2 O 1.86mg, ZnSO 4 ·7H 2 O0.22mg, Na 2 MoO 4 2H 2 O 0.39mg, CuSO 4 ·5H 2 O 0.08 mg. Initial inoculation density OD 560 The temperature is 0.5, the culture temperature is 30±2°C, and the light is continuously illuminated. The light is irradiated from one side of the columnar bubble reactor, and the light receiving area is 0.013m 2 , light intensity 150μ...

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Abstract

The invention relates to the cultivation and accumulation of arginine by spirulina, in particular to a method of cultivating spirulina by controlling the addition of nutrient salts so as to accumulate arginine. Transfer the spirulina cells cultivated to the exponential growth phase into the sulfur-limited medium, with natural light or artificial light, cultivate the algal cells to the stationary phase, and harvest the algal cells. The arginine content and yield reach 5% to 8% of the dry weight of the algae, respectively. and 65mg / L~140mg / L, which are 2~3 times and 20%~40% higher than that of spirulina under nutrient-rich conditions. The invention realizes the rapid and efficient production of arginine-rich spirulina by photoautotrophic carbon fixation, and the involved culture method has the advantages of less nutrient salt consumption, low production cost, high yield and content of arginine obtained, and carbon emission reduction It has the advantages of process coupling and other advantages, and has good economic and environmental benefits.

Description

technical field [0001] The invention relates to accumulating arginine by cultivating spirulina, in particular to cultivating spirulina by controlling the addition of nutrient salts and adopting appropriate inoculation density, light, temperature and pH value to accumulate arginine. It can be used in the cultivation and production of arginine-rich spirulina, as well as in related fields such as food, health care products, medicine, and fine chemical manufacturing using arginine as raw material. Background technique [0002] Arginine is a semi-essential amino acid that plays important physiological, metabolic and nutritional roles in the human body. The specific functions are as follows: arginine is a component in the ornithine cycle, it can increase the activity of arginase in the liver, which is beneficial to hyperammonemia, liver dysfunction, etc.; arginine is a component of sperm protein The main ingredient has the effect of promoting the quality of sperm and improving th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/38C12N1/20C12R1/01
CPCC12N1/20C12N1/38
Inventor 薛松姚长洪刘瑞国曹旭鹏陆洪斌姜君鹏
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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