Novel molecular marker and application thereof in preparing kit for diagnosis and prognosis of head and neck cancer
A technology for molecular markers and head and neck cancer, applied in biochemical equipment and methods, microbiological measurement/testing, DNA/RNA fragments, etc., can solve the problem of not significantly improving the 5-year survival rate of head and neck cancer patients, and the resection range of head and neck cancer surgery Restricted, patients' quality of life decline and other problems, to achieve high clinical use and promotion value, characteristics of good diagnostic indicators, good diagnostic effect
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[0015] The present invention will be further elaborated below through specific examples. It should be noted that the following descriptions are only for explaining the present invention, and do not limit its content.
[0016] The method for obtaining the molecular marker of the present invention is as follows:
[0017] 1. Download 620 tRNA gene annotation information and their sequences from the GtRNAdb (http: / / gtrnadb.ucsc.edu / genomes / eukaryota / Hsapi19) database. Annotations and sequence information for newly constituted CCA-tRNAs were obtained by adding "CCA" at the 3′ end of the mature tRNA sequence. Then, starting from the 5' or 3' end of the CCA-tRNAs sequence, all fragment sequences in the range of 15-30 nt were obtained. After further merging the same sequences, 8681 fragment sequences and 9285 fragment sequences from the 5' end of CCA-tRNAs were finally obtained. A fragment sequence from the 3′ end of CCA-tRNAs. In addition, we used the bedtools tool to obtain the an...
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