Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Novel molecular marker and application thereof in preparing kit for diagnosis and prognosis of head and neck cancer

A technology for molecular markers and head and neck cancer, applied in biochemical equipment and methods, microbiological measurement/testing, DNA/RNA fragments, etc., can solve the problem of not significantly improving the 5-year survival rate of head and neck cancer patients, and the resection range of head and neck cancer surgery Restricted, patients' quality of life decline and other problems, to achieve high clinical use and promotion value, characteristics of good diagnostic indicators, good diagnostic effect

Active Publication Date: 2018-05-18
ZHEJIANG UNIV
View PDF2 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After decades of development, surgery, radiotherapy and chemotherapy have not significantly improved the 5-year survival rate of head and neck cancer patients, and due to the special anatomical site, the resection range of head and neck cancer surgical treatment is severely limited, and the quality of life of patients after treatment generally declines , often disfiguring or disabling

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Novel molecular marker and application thereof in preparing kit for diagnosis and prognosis of head and neck cancer
  • Novel molecular marker and application thereof in preparing kit for diagnosis and prognosis of head and neck cancer
  • Novel molecular marker and application thereof in preparing kit for diagnosis and prognosis of head and neck cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0015] The present invention will be further elaborated below through specific examples. It should be noted that the following descriptions are only for explaining the present invention, and do not limit its content.

[0016] The method for obtaining the molecular marker of the present invention is as follows:

[0017] 1. Download 620 tRNA gene annotation information and their sequences from the GtRNAdb (http: / / gtrnadb.ucsc.edu / genomes / eukaryota / Hsapi19) database. Annotations and sequence information for newly constituted CCA-tRNAs were obtained by adding "CCA" at the 3′ end of the mature tRNA sequence. Then, starting from the 5' or 3' end of the CCA-tRNAs sequence, all fragment sequences in the range of 15-30 nt were obtained. After further merging the same sequences, 8681 fragment sequences and 9285 fragment sequences from the 5' end of CCA-tRNAs were finally obtained. A fragment sequence from the 3′ end of CCA-tRNAs. In addition, we used the bedtools tool to obtain the an...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a novel molecular marker and application thereof in preparing a kit for diagnosis and prognosis of head and neck cancer. RNA (Ribonucleic Acid) sequences of the novel molecularmarker are shown as SEQ ID NO:1 to SEQ ID NO:48. A mathematical model for diagnosing the head and neck cancer is constructed by taking the marker disclosed by the invention as a basis, and has the advantages of high sensitivity, good specificity and good diagnosis effect; AUC can be as high as 0.981; besides, 48 tRF fragments can be used as molecular markers for typing the head and neck cancer and predicting life expectancy of patients; head and neck cancer tumor samples are clustered into four subtypes according to tRFs expression in test data; the analysis of a survival curve shows that thelife expectancies of the subtypes have significant difference. The novel tRF molecular marker disclosed by the invention has good characteristics of diagnostic indicators and higher clinical use andpopularization value, and can be effectively used for diagnosis, typing and prognosis of the head and neck cancer.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a novel molecular marker and its application in preparing a kit for diagnosis and prognosis of head and neck cancer. Background technique [0002] Head and neck cancer includes tumors originating from any tissue or organ in the head and neck except the eye, brain, ear, thyroid and esophagus, including neck tumors, ENT tumors, and oral and maxillofacial tumors (oral cancer). Since more than 90% of head and neck tumors are squamous cell carcinomas, head and neck cancers are also called head and neck squamous cell carcinomas (HNSC). Head and neck cancer is a malignant tumor with a high incidence in China and even the global cancer spectrum. It ranks sixth among common tumors. It has a high degree of malignancy and an average 5-year survival rate of less than 50%. After decades of development, surgery, radiotherapy and chemotherapy have not significantly improved the 5-...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/6886
CPCC12Q1/6886C12Q2600/112C12Q2600/118C12Q2600/158
Inventor 刘鹏渊孙喜伟陆燕周莉媛
Owner ZHEJIANG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products