Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Quality testing method of traditional Chinese medicine compound with efficacy in treating acute and chronic pyelonephritis and benign prostatic hyperplasia

A quality detection method and technology for benign prostatic hyperplasia, which are applied in measurement devices, instruments, scientific instruments, etc., can solve the problems of a single detection method, the inability to comprehensively and objectively detect the quality, the complex composition of traditional Chinese medicine preparations, etc., and achieve the effect of optimizing the conditions of mass spectrometry.

Active Publication Date: 2020-10-09
JIANGSU PROVINCIAL HOSPITAL OF TCM
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The composition of the traditional Chinese medicine preparation of the present invention is complex, relying on a single detection method, it is impossible to detect its quality comprehensively and objectively

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Quality testing method of traditional Chinese medicine compound with efficacy in treating acute and chronic pyelonephritis and benign prostatic hyperplasia
  • Quality testing method of traditional Chinese medicine compound with efficacy in treating acute and chronic pyelonephritis and benign prostatic hyperplasia
  • Quality testing method of traditional Chinese medicine compound with efficacy in treating acute and chronic pyelonephritis and benign prostatic hyperplasia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] 1, a kind of quality detection method of the Chinese medicine compound prescription that has treatment acute and chronic pyelonephritis and prostatic hyperplasia effect, it is characterized in that, comprises the following steps:

[0064] (1) Preparation of reference substance solution

[0065] Accurately weigh the reference substances of timosaponin BII, timosaponin AⅢ, cortex cortex, jatrorrhizine, berberine hydrochloride, palmatine hydrochloride, mangiferin and neomangiferin, place them in a 10mL volumetric flask, and use The volume concentration of 70% acetonitrile was fixed to the mark, and the individual controls with mass concentrations of 1006 μg / mL, 999 μg / mL, 1288 μg / mL, 1251 μg / mL, 4004 μg / mL, 1775 μg / mL, 1258 μg / mL and 1114 μg / mL were prepared Product stock solution;

[0066] Precisely draw appropriate amount of timosaponin BII reference substance stock solution, timosaponin AⅢ reference substance stock solution, cortex base stock solution, jatrorrhizine st...

Embodiment 2

[0100] Example 2 Sample Determination

[0101] The preparation method of Zishen Tongguan Capsules is as follows: take the three herbs of Phellodendron Phellodendri, Zhimu and Cinnamon;

[0102] Take 50g of cinnamon and crush it into fine powder, set aside;

[0103] Take 500g of Phellodendron cortex, crush it into coarse powder, add 65% ethanol to soak for 24 hours, then percolate, control the flow rate to 2ml / min, collect the percolate until the color is light, and set aside;

[0104] Take 500g Anemarrhena, add 2500ml65% ethanol to impregnate for 24 hours, filter the impregnation solution, add 65% ethanol to the dregs and reflux extraction twice, add 2500ml65% ethanol each time, heat and reflux for 2 hours, filter, combine the filtrate, and the filtrate and Phellodendron percolate was combined, ethanol was recovered under reduced pressure and concentrated to a thick paste. Take the thick paste, add an appropriate amount of dextrin (the amount of dextrin in the dry extract mu...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a quality detection method of a traditional Chinese medicine compound with efficacy in treating acute and chronic pyelonephritis and prostatic hyperplasia. The method comprises the following steps: (1) preparation of a reference substance solution; (2) preparation of an internal standard solution; (3) ) preparation of need testing solution; (4) linear relationship investigation; (5) detection of need testing solution. According to the structures and properties of 8 kinds of compounds such as saponins and alkaloids in the traditional Chinese medicine compound, the present invention screens out the best analytical conditions such as chromatographic column, mobile phase composition, elution program, flow rate, chromatographic column and mass spectrometry through a large number of experiments. It has been verified by multiple experiments that the present invention can simultaneously detect 8 kinds of compounds such as saponins and alkaloids. The method has high detection sensitivity and good stability, and can perform detection and evaluation objectively, comprehensively and accurately, which is very important for quality control and clinical safety. Effectiveness is important.

Description

technical field [0001] The invention relates to a quality control method of a traditional Chinese medicine preparation, in particular to a quality detection method of a traditional Chinese medicine compound with efficacy in treating acute and chronic pyelonephritis and prostatic hyperplasia. Background technique [0002] The Zishen Tongguan Capsule prepared from cinnamon, Cortex Phellodendri and Anemarrhena is an in-hospital preparation of Jiangsu Provincial Hospital of Traditional Chinese Medicine. Zishen Tongguan Capsule has the functions of nourishing the kidney and clearing heat, clearing Qi and clearing the customs in removing dampness and heat in the lower energies, and assisting in gasification of the bladder. The TCM dialectical theory of the present invention is: Anemarrhena anemarrhena in the prescription is bitter and cold, the upper part clears lung heat and reduces fire, the lower part moisturizes kidney dryness and nourishes yin; Cortex Phellodendri is bitter a...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 刘顺周琴妹倪文澎邹恺平陈晓斌管敏孙蕾
Owner JIANGSU PROVINCIAL HOSPITAL OF TCM
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com