Accurate packing and mixing device for DNA primers or probes and using method of accurate packing and mixing device

Abstract

The invention discloses an accurate packing and mixing device for DNA primers or probes and a using method of the accurate packing and mixing device. The accurate packing and mixing device mainly comprises a control cabinet, an executing mechanism, a working region component, a testing unit, a gun head frame, a working table board and a fixed table board; the executing mechanism consists of an X-axis sliding table, a Y-axis sliding table, a Z-axis sliding table and a gun axis sliding table; and the working region component comprises eight packing target boards, a gun head board, a stock solution board, a solution guiding target board and a gun head separator, and the working table board uses a layout form of taking the stock solution board as the center. An accurate, reliable and efficientengineering solution scheme is provided, and therefore, automatic accurate packing and mixing of the DNA primers or probes are realized. The labor can be saved, a troublesome manual solution suckingand beating process can be omitted, an operator only needs to use a mobile USB flash disk to guide required operation into software, previous preparation operation is omitted, and full-automatic operation is realized.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and in particular relates to a precise packaging and mixing device for DNA primers or probes and a method for using the same. Background technique [0002] In molecular biology research, primers with different base sequences and lengths need to be synthesized according to specific purposes. After the DNA primer is synthesized, it is usually placed in a standard well plate, such as a 48-well plate or a 96-well plate, and each well can contain a different primer. According to the different purposes of analysis and research, sometimes it is necessary to distribute the same synthesized primer or probe (called one) into several different centrifuge tubes or screw tubes, and sometimes it is necessary to distribute different primers or probes The needles are mixed into the same tube, usually these dispensing or mixing work is done manually by the operator with the aid of a pipette. Completin...

AI Technical Summary

Problems solved by technology

Because the primers or probes that need to be packaged or mixed are measured in microliters, the operator’s skills are very high, and it is often difficult to control the measurement deviation or fluctuation in a small range

At the same time, manual operation cannot completely avoid human errors, and it is difficult to find errors when they occur

[0003] The laboratory pipetting workstation or nucleic acid extractor in the prior art can only achieve the purpose of uniformly transferring a certain liquid from one container to another, but cannot realize the complex and complex process of subpackaging and mixing primers or probes. Sophisticated requirements

Its measurement unit is usually in the milliliter level, and the measurement accuracy is about 1%, while the subpackage or mixing of primers or probes, the measurement unit is in the microliter level, and the measurement accuracy is higher. Laboratory pipetting workstations or nucleic acid extractors Unable to meet the high precision and high stability requirements of this kind of micrometering

Moreover, the laboratory pipetting workstation or nucleic acid extractor cannot realize the automatic collection of data modules, nor can it realize the compatibility and connection with the DNA primer or probe synthesis system

Images