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A Mass Spectrometry Model for Rapid and Nondestructive Diagnosis of Breast Diseases

A fast, high-resolution mass spectrometry technology, applied in the fields of analytical chemistry, biochemistry and medicine, can solve the problems of poor specificity, poor sensitivity, low hormone content, etc., and achieve the effect of reducing patient pain, improving diagnosis speed and reducing diagnosis cost

Inactive Publication Date: 2018-12-25
CHANGCHUN UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The method used is electrochemiluminescence measurement, which has the disadvantages of poor sensitivity and poor specificity
High-resolution mass spectrometry has the characteristics of high sensitivity, rapidity, and strong anti-interference ability. Its application in clinical analysis provides a new opportunity for the determination of small molecule hormones. In the past, low-resolution tandem mass spectrometry was often used in the determination of hormones, but Due to the low content of hormones in the obtained biological samples, the use of low-resolution mass spectrometry technology cannot obtain accurate mass-to-nucleus ratios, and the low analyte content prevents multi-level fragment ions from being captured and fragmented, and cannot analyze traces in complex matrices. Accurate qualitative and quantitative analysis of hormones

Method used

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  • A Mass Spectrometry Model for Rapid and Nondestructive Diagnosis of Breast Diseases
  • A Mass Spectrometry Model for Rapid and Nondestructive Diagnosis of Breast Diseases
  • A Mass Spectrometry Model for Rapid and Nondestructive Diagnosis of Breast Diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] A total of 117 samples were collected, aged 22-65, and the height, weight, and family history of the volunteers were counted in detail. Randomly select 20 healthy people (group A), 20 patients with breast cysts (group B) and 20 breast cancer patients (group C), and use electrochemiluminescence to measure the contents of two hormones, testosterone and estradiol , there was no significant difference in the experimental results, as follows:

[0042]

[0043]

[0044] Therefore, a mass spectrometry analysis model for rapid and non-destructive diagnosis of breast diseases is established, including the following steps:

[0045] (1) Sample collection

[0046] Use 75% alcohol cotton to wipe the nipple, areola and armpit of the subjects (including healthy control group A, breast cyst group B and breast cancer group C) repeatedly for 3-4 times, and during the period of wiping, ensure Subjects swabs were collected 4-5 days after bathing. Detailed information such as age, ...

Embodiment 2

[0063] 57 routine samples not detected in embodiment 1 are carried out mass spectrometry detection analysis according to the method for embodiment 1, judge mammary gland disease standard to diagnose according to hormone ratio: I 睾酮 / I 孕酮 When 睾酮 / I 孕酮 =between 0.41-0.60, can not judge; I 睾酮 / I 孕酮 >0.60, it is judged as a patient with breast disease. Further, when I 睾酮 / I 孕酮 When unable to judge, you can use I睾酮 / I 雌二醇+雌三醇+雌酮+孕酮 for analysis, when I 睾酮 / I 雌二醇+雌三醇+雌酮+孕酮 When 睾酮 / I 雌二醇+雌三醇+雌酮+孕酮 When >0.19, it is judged as a patient with breast disease, and the specific results are as follows:

[0064]

I 睾酮 / I 孕酮

Number of samples

healthy person

<0.41

32 cases

Can't judge

0.41-0.60

6 cases

Patients with breast disease

>0.60

19 cases

[0065] Furthermore, for the samples that cannot judge the state, we performed I 睾酮 / I 雌二醇+雌三醇+雌酮+孕酮 Ratio analysis, it was found that I 睾酮 / I 雌二醇+雌三醇+雌酮+孕酮 4 samples w...

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Abstract

The invention relates to a mass spectrometry analysis model for rapid and nondestructive diagnosis of breast diseases. By using medical alcohol cotton to wipe the armpits, nipples, and areola of patients with breast diseases, and using high-resolution mass spectrometry to analyze the collected samples, find the ratio of characteristic hormone compounds, and develop a non-invasive method for breast cysts and breast cancer diseases A model for early screening. On the premise that no invasive examination (blood collection, diseased tissue) is required to obtain patient samples, the present invention can quickly and non-destructively diagnose breast diseases at different stages by using the changes in the ion intensity ratio of characteristic hormones, and improve the quality of breast diseases. Diagnosis speed, lower diagnosis cost, less pain for patients.

Description

technical field [0001] The invention relates to the fields of analytical chemistry, biochemistry, medicine and the like, and proposes a mass spectrometry analysis model for rapid screening of breast disease patients without damage. Specifically, it involves a method of obtaining diseased tissue samples that does not require invasive procedures such as blood collection and surgery for patients with breast diseases. It only needs to obtain metabolite information by swabbing the patient's body surface without damage, and combined with high-resolution mass spectrometry technology to treat patients with breast diseases. A model for painless screening. Background technique [0002] Breast cancer is the most common malignant tumor in American women. In the past 40 years, the incidence of breast cancer in Asian women has also shown an upward trend. The statistical data report published by the American Cancer Society in 2015 shows that the death rate of breast cancer ranks second am...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/62
CPCG01N27/62
Inventor 苏蕊杨洪梅陈长宝
Owner CHANGCHUN UNIV OF CHINESE MEDICINE
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