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Methods for assessing the phytotoxicity of antibiotics

A technology of phytotoxicity and antibiotics, applied in the direction of testing plants/trees, testing pharmaceutical preparations, instruments, etc., to achieve the effects of easy seed attachment, simple operation, and easy preservation

Active Publication Date: 2020-06-02
HUNAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition, there is currently a lack of research on methods and indicators related to the evaluation of toxicity in seed germination experiments and research on pollutant-sensitive seeds at home and abroad.

Method used

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  • Methods for assessing the phytotoxicity of antibiotics
  • Methods for assessing the phytotoxicity of antibiotics
  • Methods for assessing the phytotoxicity of antibiotics

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Embodiment 1

[0042] A method for evaluating antibiotic phytotoxicity, wherein the antibiotic is tetracycline hydrochloride, and the vegetable seeds are Chinese cabbage seeds of Brassicaceae Brassica, comprising the following steps:

[0043] (1) Prepare a semi-solid medium containing tetracycline hydrochloride:

[0044] (1.1) prepare salt solution, wherein the concentration of NaCl is 50mM, NH 4 The Cl concentration was 0.4 mM and the pH was adjusted to 4.7 with 0.01 M HCl.

[0045] (1.2) Agar is mixed with the salt solution obtained in step (1.1), and heated to dissolve the agar to obtain a salt solution containing agar, wherein the mass-volume ratio of the agar is 1.6% (w / v).

[0046] (1.3) Take the agar-containing salt solution obtained in step (1.2), and when the temperature drops to 50° C., divide it into 6 equal parts, and mix them with equal volumes of 0 μM, 0.02 μM, 0.2 μM, 2 μM, 20 μM and The 200 μM tetracycline hydrochloride solution was mixed evenly to obtain mixed solutions wi...

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Abstract

The invention discloses a method for evaluating phytotoxicity of antibiotics. The method comprises the following steps: sowing vegetable seeds into an antibiotic-containing semisolid medium for culture; and according to germination situations of the vegetable seeds in the culture process, acquiring germination time T50, germination number, germination number probability distribution and radicle lengths of half of the vegetable seeds, and evaluating the phytotoxicity of antibiotics by taking the acquired information as parameters. By use of sensibility of the vegetable seeds to the antibiotics,the phytotoxicity of the antibiotics is evaluated through a germination experiment of the vegetable seeds. By taking the germination time T50, the germination number, the germination number probability distribution and the radicle lengths of half of the vegetable seeds as the parameters, the phytotoxicity of the antibiotics with different concentrations can be effectively evaluated. Therefore, the method has the advantages of short test period, simple operation and the like.

Description

technical field [0001] The invention belongs to the technical field of pollutant toxicity evaluation, and relates to a method for evaluating antibiotic toxicity, in particular to a method for evaluating antibiotic phytotoxicity by using a vegetable seed germination experiment. Background technique [0002] After antibiotics enter the environment through use, they have attracted widespread attention as an emerging pollutant. Especially in the aquaculture industry, antibiotics have been added to feed in low doses for the prevention of animal diseases for a long time, and most of the antibiotics are excreted by animals in the form of original drugs, and enter the soil and water through various channels. Therefore, antibiotics, as a pseudo-persistent pollutant, persist in the environment for a long time at low concentrations. At present, the research on the ecotoxicology of antibiotics based on plant experiments is an important supplement to the evaluation of the potential risk...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/15G01N33/00
CPCG01N33/0098G01N33/15
Inventor 罗渊梁婕曾光明李晓东蒋龙波武海鹏方意龙叶淑静
Owner HUNAN UNIV
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