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Application of miRNA-4731-3p as a diagnostic marker for primary lung cancer

A technology for lung cancer and purpose, applied in the field of medical diagnosis, can solve the problems of reducing lung cancer mortality and low lung cancer sensitivity

Active Publication Date: 2021-05-25
NANHUA UNIV
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Problems solved by technology

Therefore, early detection of lung cancer can reduce the mortality rate of lung cancer, but there is still no very safe and effective method to screen high-risk groups of lung cancer. Previous lung cancer markers such as cytokeratin 21-1, tumor polysaccharide, carcinoembryonic antigen diagnosis Lung cancer is less sensitive
Some studies have reported that about 50% of pulmonary nodules found by imaging examinations are benign, and have limited effect on reducing lung cancer mortality

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  • Application of miRNA-4731-3p as a diagnostic marker for primary lung cancer
  • Application of miRNA-4731-3p as a diagnostic marker for primary lung cancer
  • Application of miRNA-4731-3p as a diagnostic marker for primary lung cancer

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[0024] The sera of 40 patients with diagnosed primary lung cancer and 40 healthy people who underwent physical examination were collected. 4ML of peripheral blood was collected from each, and informed consent was obtained. Centrifuge at room temperature, 3600r, 5 minutes, take the upper plasma, and store it in a -80°C refrigerator for later use.

[0025] The RNA extraction method is as follows. Take 200UL of the extracted plasma, add it to 1ML of TRIzol at a ratio of 1:5 and mix thoroughly, and extract total RNA according to the protocol provided by TRIzol.

[0026] For the synthesis of cDNA, according to the method of QLAGEN reverse transcription kit, the dissolved total RNA is used as a template on a common PCR machine, and the reverse transcriptase, dNTP and reaction buffer provided by the kit are added to reverse transcribe mRNA into cDNA. Store at -20°C until use.

[0027] Table 1: Composition of the reaction system

[0028] Component Volume Purified RNA...

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Abstract

The invention relates to circulating miRNA-4731-3p as a diagnostic marker for primary lung cancer and a detection method thereof. Through high-throughput screening of circulating miRNAs in the plasma of patients diagnosed with lung cancer, and comparing them with the corresponding miRNAs in the plasma of healthy subjects, we found the highly expressed MiRNA‑4731‑3p as the target of this experiment Mark. The expression level of miRNA‑4731‑3p was significantly higher than that of the control group P<0.001. ROC curve analysis showed that the efficacy of miRNA‑4731‑3p in distinguishing normal people from lung cancer patients was AUC=0.048, the sensitivity was 95.0%, and the specificity was 57.5%. Survival curve analysis showed that the higher the expression level, the shorter the survival period, on the contrary, the lower the expression level, the longer the survival period.

Description

technical field [0001] The invention belongs to the technical field of medical diagnosis, and relates to the use of miRNA-4731-3p as a diagnostic marker for primary lung cancer. Background technique [0002] Worldwide, more than 1 million people die of lung cancer every year, ranking first among all malignant tumor-related deaths, of which 80% belong to non-small cell lung cancer (NSCLC). In China, in 2009, lung cancer ranked first among malignant tumors with an incidence rate of 53.57 / 100,000 and a mortality rate of 45.57 / 100,000. Although lung cancer diagnosis and treatment technology has been continuously improved in recent years, the 5-year survival rate of lung cancer patients has increased from 15% to 16.8%, but it is still one of the tumors with the worst prognosis. Lung cancer patients are also showing a younger trend. A few years ago, most lung cancer patients were over 55 years old, and few under 45 years old. However, in recent years, lung cancer patients under 4...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6886
CPCC12Q1/6886C12Q2600/118C12Q2600/158C12Q2600/178
Inventor 左建宏文美玲吕秀
Owner NANHUA UNIV
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