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Bacillus amyloliquefaciens 2yn11 for tomato disease prevention, growth promotion, quality improvement and stress resistance and its application

A 2YN11, amyloliquefaciens spore technology, applied to Bacillus amyloliquefaciens 2YN11 and its application fields, to achieve the effects of promoting growth, protecting the environment and promoting sustainable development

Active Publication Date: 2020-12-15
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a green and environmentally friendly Bacillus amyloliquefaciens 2YN11 that can improve broad-spectrum plant disease prevention, growth promotion, and stress resistance in view of the shortcomings of the existing technology for preventing and controlling tomato diseases and resisting adverse environments.

Method used

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  • Bacillus amyloliquefaciens 2yn11 for tomato disease prevention, growth promotion, quality improvement and stress resistance and its application
  • Bacillus amyloliquefaciens 2yn11 for tomato disease prevention, growth promotion, quality improvement and stress resistance and its application
  • Bacillus amyloliquefaciens 2yn11 for tomato disease prevention, growth promotion, quality improvement and stress resistance and its application

Examples

Experimental program
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Effect test

Embodiment 1

[0027] Example 1 Screening method, morphological characteristics and physiological and biochemical characteristics of Bacillus amyloliquefaciens strain 2YN11 strain

[0028] Strain 2YN11 was obtained from a farm in Jiangsu Province, which was screened from soil in areas where tomato diseases occurred seriously. The specific method was as follows. Using the gradient dilution method, 1 g of the soil collected from the diseased area was weighed and placed in a test tube filled with 9 mL of sterile water. Vigorously shake for 5 minutes to make a soil suspension, after gradient dilution for 10-4 days, spread on LB plates, culture in the dark at 28°C incubator, pick individual colonies with different colony forms after 48 hours, purify on LB plates, and store in LB at 4°C The slope is ready for use.

[0029] The screened bacterial strains were subjected to plate antagonism experiments with several pathogenic bacteria (bacterial wilt, bacterial spot, etc.). The specific method is as...

Embodiment 2

[0031] Example 2 Molecular identification of Bacillus amyloliquefaciens strain 2YN11

[0032] After extracting the bacterial gene DNA with the Bacterial Genome Kit (Saibaisheng Company), the gyrB gene fragment was amplified from the genomic DNA with gyrB universal primers gyrB1 (5'-GAAGTCATCATGACCGTTCTGCAYGCNGGNGGNAARTTYGA-3'); gyrB2R (5'-AGCAGGATACGGATGTGCGAGCCRTCNACRTCNGCRTCNGTCAT-3') . The specific amplification system and procedure (25ul) are as follows: 1 μl each of primers 1 and 2 (10 μM); Mix: 12.5 μl; Taq: 0.5 μl. wxya 2 O: 10 μl; template: 1 μl. Pre-denaturation at 94°C for 5 minutes, denaturation at 94°C for 1 minute, annealing at 56°C for 2 minutes, extension at 72°C for 2 minutes, 35 cycles, extension at 72°C for 10 minutes, and storage at 4°C. PCR amplified products were detected by 1% agarose gel electrophoresis, 90V, 30min; EB stained for 30min, and amplified products were detected. After purification, the amplified product was connected to the pMD19-T vecto...

Embodiment 3

[0033] The preparation of embodiment 3 Bacillus amyloliquefaciens bacterial strain 2YN11

[0034] A Bacillus amyloliquefaciens 2YN11 that improves the biological control effect of various crop diseases and promotes growth and stress resistance, and contains microbial components: Bacillus amyloliquefaciens 2YN11 (preservation number: 12606). It was isolated from the rhizosphere soil of zhejiang pear orchard.

[0035] The preparation method of bacillus amyloliquefaciens 2YN11 bacterial agent is as follows:

[0036] Bacillus amyloliquefaciens 2YN11 was shaken and cultivated in LB culture medium at 28°C and 180rpm for 12-16h, then centrifuged at 6000rpm for 10min to obtain the bacterial cells, and the bacterial cells were diluted with sterilized water to make bacterial agents. The total concentration of viable bacteria in the finished bacterial agent was 1×10 9 -1×10 10 CFU / mL.

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Abstract

The invention discloses bacillus amyloliquefaciens 2YN11 aimed at disease prevention, growth promotion, quality improvement and stress resistance of tomato and application thereof. The strain is named Bacillus amyloliquefaciens (Bacillus amyloliquefaciens), and was deposited in the General Microbiology Center of China Committee for the Collection of Microbial Cultures on June 13, 2016. The strain preservation number is CGMCC NO.12606. The strain preparation has an obvious biocontrol effect on various tomato diseases of at least 47.05% after the root irrigation treatment of Bacillus amyloliquefaciens 2YN11; and the plant height and root length of the plant are obviously improved, and the plant has a certain effect Growth-promoting effect, compared with the control group, the yield of farmland crops treated with 2YN11 increased by more than 30.54%, the taste of tomato fruit was significantly improved, and various nutritional indicators were significantly improved.

Description

technical field [0001] The invention belongs to the field of plant protection, and relates to bacillus amyloliquefaciens 2YN11 aimed at disease prevention, growth promotion, quality improvement and stress resistance of tomato and application thereof. Background technique [0002] Tomato (scientific name: Lycopersicon esculentum Mill.), is an annual or perennial herbaceous plant belonging to the family Solanaceae, with a body height of 0.6-2 meters, all of which have viscous glandular hairs, strong odor, stems that are easy to fall, and leaves that are pinnately compound or pinnate Deeply lobed, peduncle 2-5 cm long, often 3-7 flowers, calyx radial, corolla radial, berry oblate or subglobose, fleshy and juicy, yellow seed, flower and fruit in summer and autumn. Tomatoes in China have been produced on a large scale since the 1960s, and the high-efficiency and energy-saving greenhouse cultivation model has been vigorously promoted since the 1980s. The period from 1990 to 2005 w...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/22A01P21/00A01P1/00C12R1/07
CPCA01N63/00C12N1/20C12N1/205C12R2001/07
Inventor 郭坚华朱凯张丽娜谢越盛
Owner NANJING AGRICULTURAL UNIVERSITY
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