Fluid flow system of flow cytometry and control method thereof

A flow cytometer and liquid flow technology, applied in the field of analytical instruments, can solve the problems of the influence of experimental accuracy, the control of the stability of the sheath fluid, and the influence of the focus width, etc., so as to facilitate cleaning and plugging , Eliminate the sheath fluid pulsation phenomenon, reduce the effect of volume

Pending Publication Date: 2018-03-09
成都索尔恩科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above-mentioned liquid flow system still does not realize the control of the stability of the sheath liquid, the feedback control of the focus of the sample liquid, and the influence of changing different gears on the focus width, and thus the impact on the accuracy of the experiment.

Method used

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  • Fluid flow system of flow cytometry and control method thereof
  • Fluid flow system of flow cytometry and control method thereof
  • Fluid flow system of flow cytometry and control method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0076] figure 1 It is a cross-sectional view of a single-chamber sheath buffer device. The sheath fluid buffer device 33 has at least one inlet and at least two outlets, wherein the inlet 333 communicates with the first sheath fluid circulation pump, the outlet 331 communicates with the flow chamber, and the outlet 332 communicates with the second sheath fluid circulation pump. The height of the outlet 331 is lower than that of the outlet 332 . The first sheath liquid circulation pump injects the sheath liquid into the buffer device, and when the liquid level is higher than the outlet 332, the excess sheath liquid is drawn out by the second sheath liquid circulation pump. The height difference between the outlet 332 and the outlet 331 determines the positional potential energy of the sheath fluid, and it remains constant. The sheath buffer is vented to atmosphere through an air filter.

[0077] figure 2 It is the schematic diagram and working flow diagram of the position ...

Embodiment 2

[0079] Such as image 3 As shown, a flow cytometer liquid flow system includes a sample liquid channel 1, a flow chamber 2, a sheath liquid channel 3 and a negative pressure source channel 4, and the sample liquid channel 1, the sheath liquid channel 3, and the negative pressure source The channels 4 are all connected to the flow chamber 2; the sample liquid channel 1 includes a liquid inlet sample needle 10 and a sample liquid flow sensor 11 arranged in sequence, and the sample liquid in the liquid inlet sample needle 11 passes through the sample liquid flow sensor 12 and then flow into the flow chamber;

[0080] The negative pressure source channel 4 includes a vacuum pump 41, a first three-way solenoid valve 42, a waste liquid container 441, and a two-position two-way solenoid valve 45 in sequence; the normally open valve ports of the vacuum pump 41 and the first three-way solenoid valve 42 connected, the normally closed valve port of the first three-way electromagnetic va...

Embodiment 3

[0083] Such as Figure 4 As shown, a flow cytometer liquid flow system includes a sample liquid channel 1, a flow chamber 2, a sheath liquid channel 3 and a negative pressure source channel 4, and the sample liquid channel 1, the sheath liquid channel 3, and the negative pressure source The channels 4 are all connected to the flow chamber 2; the sample liquid channel 1 includes a liquid inlet sample needle 10 and a sample liquid flow sensor 11 arranged in sequence, and the sample liquid in the liquid inlet sample needle 11 passes through the sample liquid flow sensor 12 and then flow into the flow chamber.

[0084] The negative pressure source channel 4 includes a vacuum pump 41, a first three-way solenoid valve 42, a waste liquid container 441, and a two-position two-way solenoid valve 45 in sequence; the normally open valve ports of the vacuum pump 41 and the first three-way solenoid valve 42 connected, the normally closed valve port of the first three-way electromagnetic v...

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PUM

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Abstract

The invention discloses a fluid flow system of a flow cytometry and a control method. The fluid flow system comprises a sample fluid passage, a sheath fluid passage, a negative pressure source passageand a flowing chamber, wherein the sample fluid passage, the sheath fluid passage and the negative pressure source passage are respectively connected with the flowing chamber; the sheath fluid passage is provided with a position potential energy stabilizing device; the position potential energy stabilizing device comprises a sheath fluid container, a sheath fluid buffer device, a first sheath fluid circulating pump and a second sheath fluid circulating pump; the sheath fluid buffer device is provided with a sheath fluid inlet and two sheath fluid outlets; an inlet of the first sheath fluid circulating pump is communicated with the sheath fluid container, and an outlet of the first sheath fluid circulating pump is communicated with the sheath fluid inlet of the sheath fluid buffer device;one sheath fluid outlet of the sheath fluid buffer device is communicated with the flowing chamber, one sheath fluid outlet of the sheath fluid buffer device is communicated with an inlet of a secondsheath fluid circulating pump, and an outlet of the second sheath fluid circulating pump is communicated with the sheath fluid container; the sheath fluid outlet connected with the second sheath fluidcirculating pump in the sheath fluid buffer device is higher than the sheath fluid outlet connected with the flowing chamber. The fluid flow system has the advantages that the sheath fluid is precisely controlled, and the pulsation of the sheath fluid is prohibited.

Description

technical field [0001] The invention belongs to the technical field of analytical instruments, and relates to biological detection, particle detection and cell analysis technologies, in particular to a flow cytometer liquid flow system and a control method thereof. Background technique [0002] Flow cytometer generally includes liquid flow system, optical system, electronics and signal processing system. The principle is: the labeled cells (or particles) to be tested are prepared into a single cell (or particle) suspension as a sample solution. Under the action of the liquid flow system, the sample liquid is wrapped by the sheath liquid, focused to form a laminar flow, and flows stably through the focused laser spot and is excited to emit fluorescence. Fluorescence is received by the detection system for further data analysis to obtain information about the sample. [0003] It can be seen from the above principles that the liquid flow system is the key factor for the stabi...

Claims

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Application Information

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IPC IPC(8): G01N15/14
CPCG01N15/1404G01N2015/1413
Inventor 周丹王刚
Owner 成都索尔恩科技有限公司
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