Fluid flow system of flow cytometry and control method thereof
A flow cytometer and liquid flow technology, applied in the field of analytical instruments, can solve the problems of the influence of experimental accuracy, the control of the stability of the sheath fluid, and the influence of the focus width, etc., so as to facilitate cleaning and plugging , Eliminate the sheath fluid pulsation phenomenon, reduce the effect of volume
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Embodiment 1
[0076] figure 1 It is a cross-sectional view of a single-chamber sheath buffer device. The sheath fluid buffer device 33 has at least one inlet and at least two outlets, wherein the inlet 333 communicates with the first sheath fluid circulation pump, the outlet 331 communicates with the flow chamber, and the outlet 332 communicates with the second sheath fluid circulation pump. The height of the outlet 331 is lower than that of the outlet 332 . The first sheath liquid circulation pump injects the sheath liquid into the buffer device, and when the liquid level is higher than the outlet 332, the excess sheath liquid is drawn out by the second sheath liquid circulation pump. The height difference between the outlet 332 and the outlet 331 determines the positional potential energy of the sheath fluid, and it remains constant. The sheath buffer is vented to atmosphere through an air filter.
[0077] figure 2 It is the schematic diagram and working flow diagram of the position ...
Embodiment 2
[0079] Such as image 3 As shown, a flow cytometer liquid flow system includes a sample liquid channel 1, a flow chamber 2, a sheath liquid channel 3 and a negative pressure source channel 4, and the sample liquid channel 1, the sheath liquid channel 3, and the negative pressure source The channels 4 are all connected to the flow chamber 2; the sample liquid channel 1 includes a liquid inlet sample needle 10 and a sample liquid flow sensor 11 arranged in sequence, and the sample liquid in the liquid inlet sample needle 11 passes through the sample liquid flow sensor 12 and then flow into the flow chamber;
[0080] The negative pressure source channel 4 includes a vacuum pump 41, a first three-way solenoid valve 42, a waste liquid container 441, and a two-position two-way solenoid valve 45 in sequence; the normally open valve ports of the vacuum pump 41 and the first three-way solenoid valve 42 connected, the normally closed valve port of the first three-way electromagnetic va...
Embodiment 3
[0083] Such as Figure 4 As shown, a flow cytometer liquid flow system includes a sample liquid channel 1, a flow chamber 2, a sheath liquid channel 3 and a negative pressure source channel 4, and the sample liquid channel 1, the sheath liquid channel 3, and the negative pressure source The channels 4 are all connected to the flow chamber 2; the sample liquid channel 1 includes a liquid inlet sample needle 10 and a sample liquid flow sensor 11 arranged in sequence, and the sample liquid in the liquid inlet sample needle 11 passes through the sample liquid flow sensor 12 and then flow into the flow chamber.
[0084] The negative pressure source channel 4 includes a vacuum pump 41, a first three-way solenoid valve 42, a waste liquid container 441, and a two-position two-way solenoid valve 45 in sequence; the normally open valve ports of the vacuum pump 41 and the first three-way solenoid valve 42 connected, the normally closed valve port of the first three-way electromagnetic v...
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