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A method of cultivating a population of drug-resistant Cocoa beetle wasps

A technology of beetle beetle and coconut leaf, which is applied to the cultivation of drug-resistant population of beetle beetle and the field of cultivation of beetle beetle, which can solve the lethal effect and affect the long-term sustainable reproduction of parasitic bee wild populations, parasitic bee Harm and other issues, achieve the effect of high resistant individual frequency, satisfying rapid multiplication, and improving survival probability

Active Publication Date: 2018-11-06
ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although it is possible to set the interval between bee release and drug use to avoid direct killing of parasitic wasps by insecticides, insecticides with residual effects are still potentially harmful to parasitoids for a long time, and parasitoids touch them while searching for hosts These residual components will have a certain lethal effect, affecting the long-term sustainable reproduction of the wild population of parasitoids

Method used

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  • A method of cultivating a population of drug-resistant Cocoa beetle wasps
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  • A method of cultivating a population of drug-resistant Cocoa beetle wasps

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Embodiment 1

[0031] The cultivation of the anti-abamectin population of embodiment 1 Coconut leaf beetle

[0032]A large number of expanded and multiplied populations of the cocoa beetle preserved by the Institute of Environment and Plant Protection of the Chinese Academy of Tropical Agricultural Sciences were used as the initial population, and the above-mentioned cultivation method was followed. Regularly use abamectin to treat the test insects, form the selection pressure of the agent, and carry out subculture. With lethal 30% concentration, update the selection concentration every 5 generations, that is, F 0 to F 4 LC 30 16.92mg·L -1 , F 5 to F 9 LC 30 31.84mg·L -1 , F 10 to F 14 the LC 30 44.26mg·L -1 , carry out resistance cultivation by the above-mentioned direct contact method of adult bees and indirect contact method of young bees respectively, resistance multiple = LC of the test population before cultivation 50 / LC of the reared population 50 , to LC 50 Whether the...

Embodiment 2

[0038] The cultivation of the anti-beta-cypermethrin population of embodiment two Coconut leaf beetles

[0039] A large number of expanded and multiplied populations of the cocoa beetle preserved by the Institute of Environment and Plant Protection of the Chinese Academy of Tropical Agricultural Sciences were used as the initial population, and the above-mentioned cultivation method was also followed. Regularly use beta-cypermethrin to treat the test insects, form the pressure of drug selection, and carry out subculture. With a lethal 20% concentration, the selection concentration is updated every 5 generations, that is, F 0 to F 4 LC 20 1.17mg·L -1 , F 5 to F 9 LC 20 2.1mg·L -1 , F 10 to F 14 LC 20 3.08mg·L -1 , F 15 to F 19 LC 20 4.8mg·L -1 , carry out resistance cultivation by the above-mentioned direct contact method of adult bees and indirect contact method of young bees respectively, resistance multiple = LC of the test population before cultivation 50 / L...

Embodiment 3

[0044] Embodiment 3 Cultivation of acetamiprid-resistant populations of the coconut-heart beetle beetle

[0045] A large number of expanded and multiplied populations of the cocoa beetles preserved by the Institute of Environment and Plant Protection of the Chinese Academy of Tropical Agricultural Sciences are used as the initial population, and the cultivation method of the present invention is also used. Regularly use acetamiprid to treat the parasitic wasps, form the selection pressure of the agent, and carry out subculture. With lethal 30% concentration, update the selection concentration every 5 generations, that is, F 0 to F 4 LC 30 5.58mg·L -1 , F 5 to F 9 LC 30 21.66mg·L -1 , F 10 to F 14 LC 30 31.11mg·L -1 , the above-mentioned adult bee direct contact method and young bee indirect contact method respectively carry out resistance cultivation, resistance multiple = LC of the test population before cultivation 50 / LC of the reared population 50 , to LC 50 ...

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Abstract

The invention discloses a method for breeding drug-resistant tetrastichus brontispae populations. By a direct contact method, screening is performed according to the pesticide concentration based on the fixed lethal percentage so as to select surviving tetrastichus brontispae adults, and the surviving tetrastichus brontispae adults are bred to obtain next-generation larvae; an indirect contact method is performed on tetrastichus brontispae pupae containing the tetrastichus brontispae larvae, screening is performed according to the pesticide concentration based on the fixed lethal percentage, and successfully emerged adults are subcultured; the tetrastichus brontispae populations obtained after 15-20-generation breeding have high-efficiency drug resistance; in the presence of multiple typesof insecticides in a pest area, a variety of drug-resistant tetrastichus brontispae populations are released at the same time so as to resist damage of many different types of insecticides.

Description

technical field [0001] The invention belongs to the field of agriculture, and relates to a method for cultivating the beetle beetle, in particular to a method for cultivating the beetle beetle population with drug resistance. Background technique [0002] Brontispa longissima (Gestro) is a typical tropical invasive palm plant pest. It was first discovered in Haikou City, Hainan in June 2002. It has been widely distributed in 18 counties and cities in Hainan Province, as well as Guangdong, Guangxi, Fujian and Yunnan by the end of 2004. In some areas of the province, it is currently the most important pest in the palm plant industry in my country. Both adults and larvae of the cocoa beetle feed on the unexpanded epidermis of the heart leaves of hosts such as coconuts, forming long and narrow brown stripes parallel to the veins. After the heart leaves unfold, they appear as large brown necrotic stripes, causing the leaves to shrink and curl, and the heart leaves to die , causin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/033
CPCA01K67/033
Inventor 金涛林玉英韦兵彭正强温海波金启安
Owner ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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