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Method for extracting rhodioside in purification fermentation liquid by using macroporous absorbent resin

A technology of salidroside and pore adsorption, which is applied in the field of salidroside, can solve problems such as unreported methods of salidroside, and achieves the effects of environmental friendliness, high yield and simple operation.

Inactive Publication Date: 2018-02-13
TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] At present, the method of using macroporous adsorption resin to extract and purify salidroside in microbial fermentation broth has not been reported yet.

Method used

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  • Method for extracting rhodioside in purification fermentation liquid by using macroporous absorbent resin
  • Method for extracting rhodioside in purification fermentation liquid by using macroporous absorbent resin

Examples

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Embodiment 1

[0038] A method for extracting and purifying salidroside in a fermented liquid using a macroporous adsorption resin, comprising the following steps:

[0039] (1) Centrifuge the Escherichia coli fermentation liquid producing salidroside, and obtain the supernatant as the loading liquid;

[0040] (2) Add 2.5L of the sample solution to the SPB825L macroporous adsorption resin column with the above sample flow rate at 2BV / h;

[0041] (3) Add 1L of water to the macroporous adsorption resin column, elute through the macroporous adsorption resin column with a flow rate of 1BV / h, and remove residual supernatant and polar impurities in the macroporous adsorption resin column;

[0042] (4) With a flow rate of 1BV / h, add 1500mL of ethanol aqueous solution with a volume concentration of 20% to the macroporous adsorption resin column for elution, and collect 300-1200mL of this eluate;

[0043] (5) concentrating the eluate and drying to obtain crude salidroside;

[0044] (6) dissolving the ...

Embodiment 2

[0058] A method for extracting and purifying salidroside in a fermented liquid using a macroporous adsorption resin, comprising the following steps:

[0059] (1) Centrifuge the Escherichia coli fermentation liquid producing salidroside, and obtain the supernatant as the loading liquid;

[0060] (2) Add 1.5L of the sample solution to the AB-8 macroporous adsorption resin column with the above sample flow rate as 1BV / h;

[0061] (3) Add 1L of water to the macroporous adsorption resin column, and elute through the macroporous adsorption resin column with a flow rate of 0.5BV / h to remove residual supernatant and polar impurities in the macroporous adsorption resin column;

[0062] (4) With a flow rate of 0.5BV / h, add 2000mL of ethanol solution with a volume concentration of 10% to the macroporous adsorption resin column for elution, and collect 500-1600mL of this eluate;

[0063] (5) concentrating the eluate and drying to obtain crude salidroside;

[0064] (6) dissolving the cru...

Embodiment 3

[0067] A method for extracting and purifying salidroside in a fermented liquid using a macroporous adsorption resin, comprising the following steps:

[0068] (1) Centrifuge the Escherichia coli fermentation liquid producing salidroside, and obtain the supernatant as the loading liquid;

[0069] (2) Add 2L of the sample solution into the HPD-100C macroporous adsorption resin column at a flow rate of 3BV / h;

[0070] (3) Add 1L of water to the macroporous adsorption resin column, elute through the macroporous adsorption resin column with a flow rate of 2BV / h, and remove residual supernatant and polar impurities in the macroporous adsorption resin column;

[0071] (4) With a flow rate of 2BV / h, add 2000mL of ethanol solution with a volume concentration of 15% to the macroporous adsorption resin column for elution, and collect 400-1300mL of this eluate;

[0072] (5) concentrating the eluate and drying to obtain crude salidroside;

[0073] (6) dissolving the crude salidroside in a...

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Abstract

The invention discloses a method for extracting rhodioside in purification fermentation liquid by using macroporous absorbent resin. The method comprises the following steps of (1) performing centrifugation on bacillus coli fermentation liquid producing rhodioside to obtain supernatant fluid being sampling liquid; (2) adding the sampling liquid into a large pore resin absorption column; (3) addingwater into the large pore resin absorption column; removing remained supernatant fluid and polar impurities in the large pore resin absorption column; (4) adding an ethanol water solution with the volume concentration being 10 to 20 percent into the large pore resin absorption column for elution; collecting elution liquid accounting for 20 to 80 percent of the volume of the ethanol water solution(5) concentrating and drying elution liquid to obtain a rhodioside crude product; (6) dissolving the rhodioside crude product by the 90-percent ethanol water solution; performing crystallization; (7)repeating the step (6) to obtain the rhodioside with the purification being 98 percent or higher. The rhodioside is obtained through extraction and purification from the bacillus coli fermentation liquid producing rhodioside; the method has the advantages that the operation is simple, the yield is high, and the environment-friendly effect is achieved. The obtained rhodioside has high purity and is suitable for industrial production.

Description

technical field [0001] The present invention relates to the extraction and purification of fermentation products, in particular to a method for extracting and purifying salidroside in fermentation liquid by using a macroporous adsorption resin. Background technique [0002] Rhodiola rosea is a rare wild plant that grows in the alpine and pollution-free areas. It is a traditional medicine of the Tibetan people in my country. It has been used for more than 1,000 years. It has the functions of stimulating the nervous system, increasing work efficiency, eliminating fatigue and preventing altitude sickness. In addition, Rhodiola also has the functions of protecting cardiovascular and cerebrovascular, nerve cells, anti-tumor and anti-radiation. The main medicinal active ingredient of Rhodiola rosea is salidroside and its aglycon tyrosol. In recent years, there are more and more products such as pharmaceuticals, beverages, food and cosmetics produced with salidroside as the main r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H1/06C07H15/18
CPCC07H1/06C07H15/18
Inventor 刘涛庄以彬何庆林
Owner TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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