Dopamine detection method based on ytterbium and thulium doped sodium yttrium tetrafluoroupconverting nanoparticles

A technology of sodium tetrafluoroyttrium and nanoparticles, which is applied in the field of biological analysis and detection, can solve problems such as addiction and affect emotions, and achieve the effects of simple steps, reduced use of expensive instruments, and convenient operation

Inactive Publication Date: 2020-04-10
TIANJIN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The role of dopamine is to transmit excitement and joyful information, which can affect a person's mood. Excessive dopamine secreted in the human body can keep people excited. Therefore, dopamine can treat depression, but it can also make people addicted, resulting in dependence become an addict

Method used

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  • Dopamine detection method based on ytterbium and thulium doped sodium yttrium tetrafluoroupconverting nanoparticles
  • Dopamine detection method based on ytterbium and thulium doped sodium yttrium tetrafluoroupconverting nanoparticles
  • Dopamine detection method based on ytterbium and thulium doped sodium yttrium tetrafluoroupconverting nanoparticles

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Effect test

Embodiment 1

[0030] Example 1: Preparation and characterization of ytterbium- and thulium-doped sodium tetrafluoroyttrium up-conversion materials.

[0031](1) Weigh Y with masses of 0.0750g, 0.0350g and 0.0005g respectively 2 o 3 , Y b2 o 3 , T m2 o 3 , put these three kinds of rare earth oxides into small beaker A, respectively take 1 mL of high-purity water and 2 mL of concentrated nitric acid (63wt%) into the small beakers containing rare earth oxides, put them into an electric heating mantle and heat for nitration for 2 h, wait After the nitrification of the oxide is complete, add 4mL of high-purity water to it, and when it is quickly evaporated to dryness, continue to add 4mL of high-purity water and then evaporate to dryness until the pH of the solution is close to neutral, then evaporate to dryness for later use.

[0032] (2) Weigh 0.45g of sodium hydroxide and 0.4g of ammonium fluoride into beaker B, and weigh 0.25g of polyethyleneimine (PEI, number average molecular weight 50...

Embodiment 2

[0034] Example 2: Detection of dopamine by using the ytterbium and thulium-doped sodium tetrafluoroyttrium up-conversion material prepared above

[0035] The aqueous solution of the uniformly dispersed ytterbium and thulium-doped sodium tetrafluoroyttrium up-conversion material prepared above was fixed to volume, and then 1mL was measured and dried to obtain the mass of the solid phase substance, which can be converted into a uniformly dispersed ytterbium- and thulium-doped The concentration value of the up-conversion material in the aqueous solution of sodium tetrafluoroyttrium up-conversion material, and the aqueous solution of uniformly dispersed ytterbium and thulium-doped tetrafluoro-yttrium sodium up-conversion material as ytterbium and thulium-doped tetrafluoroyttrium sodium on Conversion material (NaYF 4 : Y b / T m ) mother solution (stock solution), take 100μL NaYF 4 : Y b / T m The stock solution, diluted to 50-120μg / mL up-conversion material solution.

[0036] ...

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Abstract

The invention discloses a dopamine detection method based on ytterbium-thulium-doped yttrium sodium tetrafluoride up-conversion nanoparticles. According to the present invention, the nanoparticles have the particle size of 5 nm and the luminescence wavelength of 473 nm, have advantages of good water solubility, good stability and simple synthetic steps, can be directly applied without labeling during the dopamine detection, and further has advantages of no requirement of modification, convenient operation and simple step, such that the use of the expensive instrument in the detection is reduced, the time-consuming is short, the detection cost is reduced, and the detection limit is 0.2 [mu]M.

Description

technical field [0001] The invention belongs to the technical field of biological analysis and detection, and in particular relates to a method for synthesizing up-conversion nanoparticles with good water solubility and small particle size and its application to the detection of small biological molecules dopamine. Background technique [0002] Up-conversion luminescence is a process in which low-energy light (usually near-infrared light at 980nm) is excited to emit high-energy light through continuous multi-photon absorption and energy transfer processes, so it is also called up-conversion. Rare earth up-conversion luminescent materials exhibit excellent performance due to the rich energy level structure of rare earth elements, such as large anti-Stokes shift, narrow emission band, long fluorescence lifetime and no background fluorescence, low toxicity and strong penetrating power Such advantages have attracted extensive attention of researchers. In recent years, up-conver...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
CPCG01N21/6428G01N21/6486
Inventor 李妍赵兵李欣格
Owner TIANJIN NORMAL UNIVERSITY
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