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Method for preparing cigarette radix angelicae sinensis extract by virtue of immobilized lactobacillus

A technology of angelica extract and lactic acid bacteria is applied in the preparation field of preparation of angelica extract for tobacco, and achieves the effects of strong anti-toxicity, increased medicinal herb aroma, and easy solid-liquid separation

Inactive Publication Date: 2018-02-09
WUHAN HUANGHELOU FLAVOR & SPICES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But there are no reports of using angelica extract

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  • Method for preparing cigarette radix angelicae sinensis extract by virtue of immobilized lactobacillus

Examples

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Effect test

Embodiment 1

[0021] Embodiment 1, a kind of method that immobilized lactic acid bacteria prepares the Angelica sinensis extract for tobacco, comprises the following steps:

[0022] (1) Add 5 times of water by weight to the chopped Angelica sinensis, reflux extraction at 100°C for 2 times, each extraction time is 2 hours, cool to room temperature after extraction, and filter to obtain the extract, combine the two The second extraction solution was used to obtain the Angelica sinensis extract A, and the conditions for the two extractions were exactly the same;

[0023] (2) Mix the angelica extract A with the PVA-SA immobilized lactic acid bacteria pellets prepared by the above method according to the mass ratio of 5:1, ferment for 1d at a temperature of 30°C and a stirring speed of 5rpm, and then place at 4°C Store in the freezer for 7 days;

[0024] (3) Filter the fermented liquid after step (2) fermentation to obtain a clarified fermented liquid, add 95% alcohol solution 3 times its mass,...

Embodiment 2

[0026] Embodiment 2, a kind of method that immobilized lactic acid bacteria prepares the Angelica sinensis extract for tobacco, comprises the following steps:

[0027] (1) Take chopped angelica, add 8 times its weight of water, reflux extraction at 98°C for 3 times, each extraction time is 3 hours, cool to room temperature after extraction, and filter to obtain the extract, combined three times Extraction solution, obtain Angelica sinensis extract A; The conditions of three extractions are exactly the same;

[0028] (2) Mix Angelica sinensis extract A with the PVA-SA immobilized lactic acid bacteria pellets prepared in the above method according to the mass ratio of 8:1, and ferment for 2 days at a temperature of 35°C and a stirring speed of 8rpm, and then Store in a 4°C freezer for 6 days;

[0029] (3) Filter the fermented liquid after step (3) fermentation to obtain clarified fermented liquid, add 80% alcohol solution with 4 times its quality, precipitate for 48 hours, filt...

Embodiment 3

[0031]Embodiment 3, a kind of method that immobilized lactic acid bacteria prepares the extract of Radix Angelica sinensis for cigarettes, comprises the following steps:

[0032] (1) Take the chopped Angelica sinensis, add water 5 times its weight, reflux extraction at 95°C for 3 times, each extraction time is 4 hours, cool to room temperature after extraction, and filter to obtain the extract, combine three times extracting solution, to obtain Angelica extract A; three extraction conditions are exactly the same;

[0033] (2) Mix the Angelica sinensis extract A with the PVA-SA immobilized lactic acid bacteria pellets prepared by the above method at a mass ratio of 10:1, ferment for 1.5 days at a temperature of 25°C and a stirring speed of 10 rpm, and then place them in 4 Store in a freezer for 5 days

[0034] (3) Filter the fermented liquid after step (2) fermentation to obtain a clarified fermented liquid, add 95% alcohol solution of 2 times its mass, precipitate for 48 hour...

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Abstract

The invention provides a method for preparing a cigarette radix angelicae sinensis extract by virtue of immobilized lactobacillus. Specifically, the preparation method comprises the following steps: (1) conducting water extraction on prepared radix angelicae sinensis at 95-100 DEG C for several times, and combining extracting solutions, so that a radix angelicae sinensis extract A is obtained; (2)inoculating a broth medium with immobilized lactobacillus, conducting culture and centrifuging, so that a wet bacterium is obtained; adding the wet bacterium to a mixed solution of polyvinyl alcoholand sodium alginate, adding the mixed solution to a boric acid saturated solution containing 1-3% of CaCl2 drop by drop by virtue of a peristaltic pump and conducting cross-linking, so that PVA-SA immobilized lactobacillus spheres are obtained; and (3) mixing the radix angelicae sinensis extract A and the immobilized lactobacillus spheres at the mass ratio of (5-10) to 1 and conducting fermentation, then conducting filtering so as to obtain clear fermentation liquid, adding an alcohol solution and promoting precipitation, conducting filtering and concentrating supernatant liquid, so that the radix angelicae sinensis extract is obtained. The cigarette radix angelicae sinensis extract provided by the invention, when added to cigarettes, can enrich cigarette fragrance, improve smell of medicinal herbs and sour aroma, improve the permeability of the cigarette fragrance, enhance the feeling of generating saliva of smoke, offer soft and delicate smoke, reduce irritation, improve throat comfort and mask offensive odor.

Description

technical field [0001] The invention relates to the field of tobacco additives, in particular to a preparation method for preparing tobacco angelica extract by using immobilized lactic acid bacteria. Background technique [0002] Flavoring is a key link in the cigarette process, and it is one of the effective means to improve the determinant of product style and improve the quality of cigarette aroma. At present, flavors and fragrances for tobacco are mainly prepared through extraction and chemical synthesis of natural animals and plants. Due to limited natural plant resources and harsh conditions for chemical synthesis. With the development of biotechnology, in recent years, the use of microbial fermentation to prepare tobacco flavor raw materials provides an economical and efficient way for the production of tobacco flavor raw materials, and develops some new types of tobacco flavor raw materials that cannot be produced by traditional methods. [0003] Microbial immobili...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A24B15/20A24B3/12
CPCA24B15/20A24B3/12
Inventor 庞登红尹团章黄龙潘妮苏宏
Owner WUHAN HUANGHELOU FLAVOR & SPICES
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