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Primer pair for identifying dendrobium huoshanense and dendrobium candidum and application thereof

A technology of Dendrobium huoshanensis and Dendrobium officinale, which is applied in the field of molecular biology, can solve the problems of long time consumption, unfavorable realization of rapidity, on-site detection, etc., and achieves the effects of short time consumption, low requirements for instruments and equipment, and simple operation process.

Inactive Publication Date: 2018-01-23
WEST ANHUI UNIV
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

Li Dan, Li Zhenjian, etc., published the identification and phylogenetic analysis of Dendrobium materials based on ITS sequences, Acta Horticultural Science, 2012, 39(8):1539-1550, using DNA barcode ITS sequences to identify Dendrobium huoshanense and its related species , but this method requires sequencing, which takes a long time and uses large instruments, which is not conducive to rapid and on-site detection

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  • Primer pair for identifying dendrobium huoshanense and dendrobium candidum and application thereof
  • Primer pair for identifying dendrobium huoshanense and dendrobium candidum and application thereof
  • Primer pair for identifying dendrobium huoshanense and dendrobium candidum and application thereof

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Embodiment Construction

[0022] The present invention will be further explained below in conjunction with the accompanying drawings and specific embodiments.

[0023] Accompanying drawing of the present invention figure 1 Above the middle pedigree branch node is the posterior probability PP of the BI tree and the bootstrap support BS of the MP tree, the PP value is on the left side of the slash, and the BS value is on the right side of the slash;

[0024] figure 2 Middle M: ​​DNA molecular standard (DNA Marker, 2000, 1000, 750, 500, 250 and 100bp from top to bottom), 1-Dendrobium huoshanense, 2-Dendrobium candidum, 3-blank control;

[0025] image 3 Middle M: ​​DNA molecular standard (DNA Marker, 2000, 1000, 750, 500, 250 and 100bp from top to bottom), 1-the result of amplifying a Dendrobium huoshanense sample with specific PCR primer pair SH-CP25, 2-the result of amplifying a Dendrobium officinale with specific PCR primers SH-CP25, 3-blank control;

[0026] Figure 4 Middle M: ​​DNA molecular s...

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Abstract

The invention discloses a primer pair for identifying dendrobium huoshanense and dendrobium candidum. The primer pair consists of a primer SEQ ID No. 1 single-stranded DNA molecule and a primer SEQ IDNo. 2 single-stranded DNA molecule, wherein the SEQ ID No. 1 single-stranded DNA molecule is a single-stranded DNA molecule in a sequence 1 in a sequence table, and the SEQ ID No. 2 single-stranded DNA molecule is a single-stranded DNA molecule in a sequence 2 in the sequence table. According to the invention, through the specific primer pair, including the primer SEQ ID No. 1 single-stranded DNAmolecule and the primer SEQ ID No. 2 single-stranded DNA molecule, rapid and accurate identification between the dendrobium huoshanense and the dendrobium candidum is achieved by using a rapid PCR method, thereby providing a new method for identification of the dendrobium huoshanense.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular to a primer pair for identifying Dendrobium Huoshanense and Dendrobium candidum and its application. Background technique [0002] Dendrobium huoshanense (Dendrobium huoshanense) is a unique plant in the Dabie Mountains of Anhui Province, and is a precious Chinese herbal medicine under national key protection, belonging to the genus Dendrobium (Orchidaceae). Dendrobium catenatum is a closely related species of Dendrobium huoshanense. The morphological characteristics of the two are relatively similar. After making a maple bucket, it is difficult to distinguish the two through their appearance. As a result, Dendrobium candidum has become one of the confused products of D. huoshanense in the market. Because it is difficult to identify Dendrobium Huoshanense and Dendrobium candidum by appearance, the traditional identification method has certain limitations. Therefore, it is...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11
Inventor 赵群孙传伯陈乃富韩邦兴陈存武
Owner WEST ANHUI UNIV
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