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Pronucleus microinjection method for transgenic rabbit model manufacturing process

A technology of microinjection and model manufacturing, applied in the field of transgenic rabbit models, can solve the problem of lack of CETP in mice and rats

Inactive Publication Date: 2018-01-12
SUZHOU HUQIAO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] (3) Mice and rats are born lacking CETP

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0010] Embodiment: When cultivating the transgenic rabbit model, at first superovulation and synchronous estrus (injection of pregnant horse serum gonadotropin or follicle-stimulating hormone) induce superovulation of the donor female rabbit, and after mating with the male rabbit (17~20 Hours later), the donor female rabbit was killed, the abdominal cavity was opened, the oviduct was taken out, and the fertilized eggs were washed and collected (the fertilized eggs with normal morphology were collected and cultured together until they were used for microinjection) and then the exogenous gene was injected into the fertilized eggs. In the male pronucleus (under 200 times magnification, under the inverted differential interference contrast microscope with a mechanical wall, the fertilized egg is sucked with a fixed needle, and the exogenous solution sucked into the injection needle is injected into the male pronucleus) after injection The embryos were retransplanted into pseudopreg...

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Abstract

The invention discloses a pronucleus microinjection method for a transgenic rabbit model manufacturing process. The pronucleus microinjection method comprises the steps of under 200 magnification times, in an inverted differential interference phase contrast microscope with a mechanical wall, sucking a fertilized ovum by using a fixation needle, and injecting an exogenous solution sucked into an injection needle into a male pronucleus. The method is used for realizing an injection process of an exogenous gene.

Description

Technical field: [0001] The invention relates to the technical field of transgenic rabbit models, in particular to a pronuclear microinjection method in the process of making transgenic rabbit models. Background technique: [0002] Cardiovascular and cerebrovascular diseases are currently the most serious diseases that threaten human health. Atherosclerosis is its pathological basis. The study of CVD is inseparable from animal models. We know that the incidence of human CVD is closely related to abnormal lipid metabolism. Atherosclerosis It is the common pathological basis of all CVD, so human research on CVD is inseparable from abnormal lipid metabolism and atherosclerosis, and now there are defects in the widespread use of mouse and rat models in biomedical research to study CVD: [0003] (1) High-density lipoprotein (HDL) is the main lipoprotein in mice and rats. HDL has innate anti-atherosclerosis effect and is not easy to form atherosclerosis. [0004] (2) apoB100 in m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/89
Inventor 刘恩岐施文标
Owner SUZHOU HUQIAO BIOTECH
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