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A kind of umbilical cord stem cell anti-wrinkle agent and preparation method thereof

An umbilical cord stem cell and wrinkle-removing technology, which is applied in the field of umbilical cord stem cell wrinkle-removing agent and its preparation, can solve the problems of foreign body granulomas, difficult to achieve accurate measurement, and complicated preparation and operation of autologous fat cell transplantation for wrinkle removal, and achieves better skin Smooth, good wrinkle removal effect

Active Publication Date: 2021-05-28
深圳市沃英达生命科学有限公司
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AI Technical Summary

Problems solved by technology

There are still many problems in the biological wrinkle removal technology currently used. For example, botulinum toxin type A is the most poisonous drug so far. The quality of the botulinum toxin used makes it difficult to achieve accurate measurement; At present, due to various conditions, this injection method has not been officially used in China; collagen, due to the need for repeated filling and the possibility of foreign body granulomas, is rarely carried out in China; the duration of hyaluronic acid is consistent with that of collagen, only 3 -6 months, continuous injections are required; the preparation and operation of autologous fat cell transplantation for wrinkle removal are cumbersome
The existence of these technical deficiencies limits the use of current rhytidectomy

Method used

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  • A kind of umbilical cord stem cell anti-wrinkle agent and preparation method thereof

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Experimental program
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Effect test

preparation example Construction

[0024] A preparation method of an umbilical cord stem cell wrinkle-removing agent, comprising the following steps:

[0025] (1) Isolation and culture of umbilical cord stem cells

[0026] S1. Using DMEM / F12 as the base medium, add platelet lysate, bFGF, and L-glutamine, so that the final concentrations of platelet lysate, bFGF, and L-glutamine are 5%, 50ng / ml, and 4mM / ml, respectively. ml, store in dark place at 4°C for later use;

[0027] S2. Transfer the umbilical cord tissue to a sterile collection bottle and store it at 2-8 degrees Celsius;

[0028] S3. Take out the umbilical cord from the umbilical cord collection bottle, soak and wash it, then use a scalpel to cut off the part of the bilateral surgical line ligation and congestion, and then rinse it with normal saline containing double antibodies (1% penicillin and 1% streptomycin) for 3 times , to remove blood stains;

[0029] S4. Put the cleaned umbilical cord in a large petri dish, divide it into several parts, eac...

Embodiment 1

[0037] Test design The effect of umbilical cord stem cell wrinkle remover and other wrinkle remover is compared. In this example, the density of umbilical cord stem cells is 2×10 7 per mL, the concentrations of hyaluronic acid, collagen, and apple proanthocyanidins were 3 mg / mL, 12 mg / mL, and 6 mg / mL, respectively. Wherein the umbilical cord stem cells are the third to fifth generation umbilical cord stem cells.

[0038]Divide 200 female subjects aged 30-60 into 4 groups, with 50 people in each group, and the average age of each group is 40.5±5.6. The first group uses the anti-wrinkle agent of this embodiment, and the second group uses Botulinum toxin type A was used as an anti-wrinkle agent, the third group used hyaluronic acid as an anti-wrinkle agent, and the fourth group used hyaluronic acid and platelet-rich high-concentration plasma as an anti-wrinkle agent. The faces of the four groups of subjects were injected with 5 mL of the preparation, observed continuously for 60...

Embodiment 2

[0049] Design experiments to evaluate the effect of different concentrations of umbilical cord stem cells to prepare anti-wrinkle agents,

[0050] In this example, the density of umbilical cord stem cells was 2×10 7 pcs / mL, 3×10 7 / mL and 4×10 7 pcs / mL, the concentrations of hyaluronic acid, collagen, and apple proanthocyanidins are 3mg / mL, 12mg / mL, and 6mg / mL, respectively. The umbilical cord stem cells are the third to fifth generation umbilical cord stem cells.

[0051] Divide 150 female subjects aged 30-60 into 4 groups, with 50 people in each group, the average age of each group is 42.5±5.6, the first group uses anti-wrinkle agent: the density of umbilical cord stem cells is 2× 10 7 pcs / mL, the concentrations of hyaluronic acid, collagen, and apple proanthocyanidins were 3mg / mL, 12mg / mL, and 6mg / mL, respectively; 7 each / mL, the concentrations of hyaluronic acid, collagen, and apple proanthocyanidins were 3mg / mL, 12mg / mL, and 6mg / mL respectively; 7 pcs / mL, the concen...

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Abstract

The invention discloses a wrinkle-removing agent for umbilical cord stem cells, which comprises the following components: umbilical cord stem cells, hyaluronic acid, collagen and apple proanthocyanidins. A preparation method of an umbilical cord stem cell anti-wrinkle agent, comprising the following steps: (1) separating and culturing the umbilical cord stem cells, and (2) completing the preparation. The anti-wrinkle agent of the present invention has a better anti-wrinkle effect through the synergistic effect of umbilical cord stem cells, hyaluronic acid, collagen, and apple proanthocyanidins, wherein 3×10 7 The anti-wrinkle formula of umbilical cord stem cells / mL and 8mg / mL apple proanthocyanidins has the best effect.

Description

technical field [0001] The invention relates to the technical field of beauty cosmetics, in particular to an umbilical cord stem cell wrinkle remover and a preparation method thereof. Background technique [0002] In the life process of organisms, aging is unavoidable, and a series of appearance changes, internal tissue changes and aging-related diseases accompanied by aging bring great trouble and inconvenience to normal life activities. In the process of aging, the changes reflected in the face are from full luster in youth to sunken wrinkles in old age, and the tissue elasticity is gradually weakened, which makes the facial appearance seriously decline, which is mainly manifested as the increase of wrinkles. With the development of biological science and the advancement of medicine, people have made some progress in anti-aging, and the changes to facial aging are mainly reflected in wrinkle removal. Rhytidectomy is mainly a series of treatments for the loosening of facia...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K8/99A61K8/49A61K8/65A61K8/73A61Q19/08C12N5/0775
CPCA61K8/498A61K8/65A61K8/735A61K8/99A61Q19/08C12N5/0665C12N2509/00
Inventor 刘俊李陶林词雄王旭林洁璇朱刚
Owner 深圳市沃英达生命科学有限公司
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