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A kind of extraction method of sesquiterpene compound

An extraction method and technology for sesquiterpenoids are applied in the field of extraction of sesquiterpenoids, and can solve problems such as undiscovered information related to Plumeria japonica and sesquiterpenoids, and achieve simple and easy extraction methods, easy-to-obtain reagents, and the like. Pain relief effect

Active Publication Date: 2019-12-24
INNER MONGOLIA UNIV FOR THE NATITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, after a lot of searching, no information about Prunus chinensis and sesquiterpenoids has been found

Method used

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  • A kind of extraction method of sesquiterpene compound
  • A kind of extraction method of sesquiterpene compound
  • A kind of extraction method of sesquiterpene compound

Examples

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Embodiment 1

[0025] Implementation Example 1: Extracting sesquiterpenoids with Prunus japonica as raw material. Plum grass (Parnassia palustrisLinn.), the whole herb or root of the saxifrage plant Plum grass, Mongolian medicine is called Menggendigeda, Mongolian name Uledige, bitter, rough, cool, with It has the functions of clearing away heat, suppressing the sun, and breaking the ruffian. It is a characteristic Mongolian medicine commonly used by the Mongolian people in the past dynasties and among the people.

[0026] Extraction method: Take 5.0kg of Prunus japonica, add 50L of 95% ethanol, reflux for extraction for 5 hours, filter, extract the dregs with 30L of 95% ethanol twice, filter, combine the filtrate, recover the ethanol from the filtrate under reduced pressure, and obtain the total extraction 1650g of the product, and the total extract was extracted with petroleum ether, dichloromethane, ethyl acetate, and n-butanol successively. Among them, 60g of dichloromethane extract was...

Embodiment 2

[0027] Implementation Example 2: Extracting new sesquiterpenoids from Prunus chinensis.

[0028]Extraction method: Take 5.0kg of Prunus japonica, add 50L of 95% ethanol, reflux for extraction for 5 hours, filter, extract the dregs with 30L of 95% ethanol twice, filter, combine the filtrate, recover the ethanol from the filtrate under reduced pressure, and obtain the total extraction Extract 1650g, the total extract is extracted with petroleum ether, dichloromethane, ethyl acetate, n-butanol successively, wherein, dichloromethane extract 60g, add 10g silica gel 100-200 order mix sample, fully dry, go up silica gel column chromatography Separation, petroleum ether-acetone gradient elution, combined into 8 fractions by TLC: No. 1 fraction: after petroleum ether elution, petroleum ether: acetone = 15:1 as developer, the same spot after TLC The materials combined; No. 2-3 fractions: petroleum ether: acetone=80:1 elution, petroleum ether: acetone=15:1 as developer, after TLC inspect...

Embodiment 3

[0029] Implementation Example 3: Extracting new sesquiterpenoids from Prunus chinensis.

[0030] Extraction method: Take 5.0kg of Plum Blossom Grass, add 50L of 95% ethanol, reflux for 5 hours, and filter. The dregs were extracted twice with 30L of 95% ethanol for 2 hours each time, filtered, and the filtrates were combined. Ethanol was recovered from the filtrate under reduced pressure to obtain 1200 g of the total extract. The total extract was added with water to form a suspension, which was sequentially extracted with petroleum ether, dichloromethane, ethyl acetate and n-butanol. Add water to the dichloromethane extract to make a suspension, put the suspension on a macroporous resin chromatographic column, first elute with 45% ethanol, then elute with 95% ethanol, combine the 95% ethanol eluent, and recover under reduced pressure ethanol. The residue was dissolved in chromatographic methanol, and the solution was the test solution. The chromatographic column is EZ0566,...

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Abstract

The invention relates to a compound, namely, novel sesquiterpenoids as well as an extraction method and an application thereof, in particular to novel sesquiterpenoids extracted from a Mongolian medicine, namely, parnassia palustris. The chemical name of novel sesquiterpenoids is 3,4,9,12-tetraacetyl-6-benzoyl-10,13 epoxy-amine alkane. The extraction method of novel sesquiterpenoids comprises steps as follows: the parnassia palustris is taken, subjected to reflux extraction with ethanol and filtered, a filtrate is obtained, ethanol is recovered from the filtrate under reduced pressure, a total extract is obtained and extracted with dichloromethane, an extract and silica gel are blended, the mixture is sufficiently dried and subjected to chromatographic separation on a silicagel column and gradient elution through petroleum ether-acetone, eluates are mixed to form 8 fractions through thin-layer examination, the fraction 6 is separated with a chromatography method, and 3,4,9,12-tetraacetyl-6-benzoyl-10,13 epoxy-amine alkane is obtained. The novel sesquiterpenoids have the benefits as follows: the sesquiterpenoids with the anti-inflammatory activity, the antioxidant activity, other activities and particularly the antitumor acidity are obtained, the extraction method is simple and easy to implement, reagents are easy to obtain, material consumption is low, and the sesquiterpenoids are expected to relieve pain of numerous cancer patients, so that the cancer patients regain health.

Description

technical field [0001] The invention relates to a compound, that is, a method for extracting sesquiterpene compounds, in particular to extracting sesquiterpene compounds from Mongolian medicine Meihuacao. Background technique [0002] Sesquiterpenoids are widely found in plants, marine organisms, certain insects and fungi. Studies have shown that sesquiterpenoids, especially polyesterified sesquiterpenes, have strong biological activity and good medicinal value and health care. However, since the research on sesquiterpenoids involves technology and equipment at the molecular level, it is very difficult to develop new products, especially the discovery frequency of new sesquiterpenoids with specific molecular structures is extremely low. Most of the sesquiterpenoids that have been discovered have problems such as scarcity of raw materials, difficulty in extraction, high cost, etc., and it is difficult to meet the needs of the market. Therefore, finding more kinds of sesquit...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D307/94A61P35/00
CPCC07D307/94
Inventor 辛颖王青虎达拉胡
Owner INNER MONGOLIA UNIV FOR THE NATITIES
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