Anti-soybean cyst nematode-related caps marker detection method and primers

A soybean cyst nematode and soybean technology, applied in the biological field, can solve problems such as difficulty in success, inability to cope with nematode toxicity variation, long time, etc., and achieve the effects of avoiding time-consuming and labor-intensive, remarkable cultivation effect, and improving selection efficiency.

Active Publication Date: 2020-07-14
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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Problems solved by technology

Due to the genetic complexity of SCN resistance genes and the diversity of soybean cyst nematode physiological races, it takes a long time to breed soybean cyst nematode-resistant varieties using traditional breeding methods, and it is difficult to succeed and cannot cope with nematodes Toxic variation of SCN cannot satisfy the current development of line-resistant breeding, and a more accurate and efficient method for identifying SCN is urgently needed

Method used

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  • Anti-soybean cyst nematode-related caps marker detection method and primers
  • Anti-soybean cyst nematode-related caps marker detection method and primers
  • Anti-soybean cyst nematode-related caps marker detection method and primers

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Embodiment 1

[0032] Example 1. Development and application of CAPS markers related to soybean cyst nematode resistance

[0033] 1. Primer design and marker development

[0034] Download the Glyma.11G234500 gene sequence from phytozome (https: / / phytozome.jgi.doe.gov / pz / portal.html), the gene Glyma.11G234500 and its homologous gene Glyma.18G022500 have a high nucleotide sequence Therefore, in order to ensure the specificity of the primer sequence, the sequence comparison of the two genes was carried out first, and the primers were designed at the difference sites. Primer pair A was designed using Primer5.0 software. Use the online software dCAPSFinder2.0 (http: / / helix.wustl.edu / dcaps / dcaps.html) to develop SNPs into CAPS markers (the marker site information selected by the present invention is shown in Table 1), select the enzyme AciI ( Beijing Bailingke Biotechnology Co., Ltd., Cat. No.: R0551S) for enzyme digestion analysis. The expected length of the PCR amplification product of primer...

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Abstract

The invention discloses a CAPS marker detection method and a primer relevant to soybean cyst nematode resistance. The invention provides application of single nucleotide polymorphism of following SNP locus in a soybean genome or a substance for detecting the single nucleotide polymorphism of the following SNP locus in the soybean genome for identifying or assisting identification of soybean resistance to the soybean cyst nematode; the SNP locus in the soybean genome corresponds to 113th site of nucleotide sequence shown as sequence 1 in a sequence list; and nucleotide at the SNP locus is G or T. The CAPS marker detection method and the primer relevant to the soybean cyst nematode resistance can select materials resistant to the soybean cyst nematode by detecting genotype of the SNP locus, can avoid time-consuming and labor-consuming phenotypic identification, can carry out soybean cyst nematode resistance screening on breeding materials through the genotype, increase selection efficiency of resistant varieties in a breeding process and shorten the breeding time largely, and improve cultivation effects of varieties resisting the soybean cyst nematode significantly.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a method for detecting a CAPS marker related to soybean cyst nematode resistance and primers. Background technique [0002] Soybean Cyst Nematode (SCN) is an essential static parasitic nematode in roots, which causes huge losses to soybean production. In the United States alone, from 1996 to 2014, soybean cyst nematodes accounted for approximately 36% of the damage caused by various diseases on soybeans. Breeding resistant materials and incorporating crop rotations is a major approach to disease control. Due to the genetic complexity of SCN resistance genes and the diversity of soybean cyst nematode physiological races, it takes a long time to breed soybean cyst nematode-resistant varieties using traditional breeding methods, and it is difficult to succeed and cannot cope with nematodes The toxic variation of SCN cannot satisfy the current development of line-resistant breeding, and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11A01H1/02
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 邱丽娟李英慧田宇
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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