Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A haploid, corn technology, applied in the directions of botanical equipment and methods, applications, instruments, etc., can solve problems such as applicability limitations, and achieve the effect of simplifying the identification steps
Active Publication Date: 2017-10-24
CHINA AGRI UNIV
View PDF1 Cites 5 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
However, these systems for sorting haploids based on visual recognition of color have limitations in applicability due to the need for clear color expression of the kernels
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0045] Embodiment 1, the preparation of hybrid population
[0046] 1. Preparation of DH series
[0047] 1. Maize inbred line Qi 319 (female parent) was crossed with corn inbred line Chang 7-2 (male parent) to obtain hybrid offspring (grain).
[0048] 2. The hybrid progeny (plant) obtained in step 1 is used as the female parent, and is crossed with the induced line CAU5 as the male parent to obtain the hybrid progeny (grain).
[0049] 3. Select pseudo-haploids from the hybrid offspring (grains) obtained in step 2, and perform haploid doubling to obtain 107 DH lines. Named successively as DH series 1 to DH series 107.
[0050] 2. Preparation of Hybrid Population A
[0051] Each DH line obtained in step 1 is used as the female parent, and the induced line CHOI3 is used as the male parent to perform hybridization to obtain hybrid offspring (grains). 30-40 pseudo-haploids and n1 (n1=10 or 0) pseudo-diploids were randomly selected from the hybrid offspring (grain) obtained from ...
Embodiment 2
[0063] Embodiment 2, establishing a model to identify haploids by collecting infrared spectra
[0065] Each grain in the hybrid population A prepared in Example 1, the hybrid population B prepared in Example 1, and the hybrid population C prepared in Example 1 was respectively subjected to near-infrared transmission spectrum scanning (the embryo surface of the grain faces the light source, and the light source is 3 cm away from the spectrometer ). The spectrometer is a micro spectrometer MicroNIR1700 produced by JDSU Company, the spectral range is 950-1600nm, and the single measurement time is 1s. Micro spectrometer MicroNIR1700 collects near-infrared transmission spectrum absorption values at 125 specific wavelengths in the spectral range 950-1600nm.
[0066] 2. Identification of pseudo-haploid and pseudo-diploid authenticity
[0067] After step 1 is completed, each seed in the hybrid population A prepared...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
PUM
Login to view more
Abstract
The invention discloses a method for identifying maize haploids. The method for identifying maize haploids provided by the invention comprises the following steps: (1) taking whether each corn kernel in a training set is a real haploid or a real diploid as a dependent variable, taking a near-infrared transmittance spectroscopy absorption value of each corn kernel as an independent variable, and establishing a haploid identification model by using a machine learning algorithm, wherein the training set is composed of a plurality of corn kernels, one part of the corn kernels are the real haploids, and the other part of the corn kernels are the real diploids; and (2) taking to-be-detected corn kernels, acquiring the near-infrared transmittance spectroscopy absorption values, inputting the absorption values into the haploid identification model established in the step (1), and outputting the result that the to-be-detected corn kernels are predicted haploids or predicted diploids by the model. The method provided by the invention can be used for automatic identification and has significances for promoting the maize haploid breeding technique engineering.
Description
technical field [0001] The invention belongs to the field of plant breeding, and in particular relates to a method for identifying haploids of maize. Background technique [0002] Corn is the crop with the largest planting area in my country, and it is also an important food crop, which plays an irreplaceable role in the fields of national production and food security. In 2006, the planting area of corn was 26.66 million hectares. In 2009, it surpassed rice to become the crop with the largest planting area in my country. According to data from the website of the National Bureau of Statistics, in 2016, the national corn planting area was 36.76 million hectares, with a total output of 219 million tons, and the corn yield level reached 5972.7 kg / ha. The continuous improvement of the yield level of maize is inseparable from the genetic improvement of germplasm and the selection of excellent hybrids. The single-cross varieties widely used in production make maize a model of h...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to view more 
Login to view more