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A blood collection tube and its application

A technology of blood collection tubes and standard products, applied in the field of blood collection tubes, to achieve the effect of facilitating quality control management

Active Publication Date: 2019-09-13
广州基赛生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the defects of existing blood collection tubes and provide a blood collection tube that can preserve and transport free DNA in plasma at room temperature without affecting subsequent detection results, and at the same time realize quality control management

Method used

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  • A blood collection tube and its application
  • A blood collection tube and its application

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Experimental program
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Effect test

Embodiment

[0020] Preparation of quality control standards:

[0021] Chemically synthesize a single-stranded nucleic acid with a specific sequence as shown in SEQ ID NO: 1, design specific primer sequences as shown in SEQ ID NO: 2-3, and hand over to Shanghai Sangong or Life Company to synthesize double-stranded DNA. According to the synthesized The number of DNA molecules was serially diluted with deionized water, and the working liquid diluted to 1 pmol / μL was used as a quality control standard, and 1 μL of working liquid was added to each blood collection tube.

[0022] Preparation of stabilizer protectant:

[0023] Weigh the following items into the beaker, then weigh 70ml of deionized water into the beaker with a measuring cylinder:

[0024]

[0025] Thoroughly stir and mix the above solution, filter and sterilize it with a disposable filter with a pore size of 40 microns, and add 300 μL to each blood collection tube.

[0026] Preparation of blood collection tubes:

[0027] Th...

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Abstract

The invention discloses a blood-collecting tube and application thereof. In the blood-collecting tube, one section of DNA serves as a quality control standard product and the nucleotide sequence is as shown in SEQ ID NO:1; in the blood-collecting tube, a stable protective agent is provided, and the stable protective agent comprises a formaldehyde releasing agent, a preservative, an anticoagulant and chlorate. According to the blood-collecting tube provided by the invention, stable preservation of free DNA in samples, particularly plasma can be realized, the performance of the blood-collecting tube can be monitored effectively for a long time, hemolysis factors in the samples can be checked, quality control management on the blood-collecting tube can be facilitated, the problems caused by unstable quality of the blood-collecting tube are avoided, influence on the subsequent detection results is avoided, and the blood-collecting tube can be applied to biological detection in various fields of laboratories, medical treatment and the like and has a good application value.

Description

technical field [0001] The invention belongs to the technical field of biological detection. More specifically, the invention relates to a blood collection tube capable of storing and transporting free DNA in plasma at room temperature without affecting subsequent detection results, and at the same time realizing quality control management. Background technique [0002] With the rapid development of next-generation gene sequencing technology, non-invasive prenatal diagnosis based on gene sequencing technology has become a powerful means in the field of fetal genetic disease diagnosis. Non-invasive prenatal diagnosis mainly uses free fetal DNA in pregnant women's plasma for specific prenatal screening and diagnosis. It is easy to be polluted by chromosomal DNA released by the rupture of maternal nucleated cells and degraded by high concentration of DNase in the cells. [0003] At present, in the detection of free DNA, such as cfDNA and ctDNA, special blood collection tubes a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/34C12M1/24C12Q1/686C12N15/10A61B5/15
Inventor 张镜悬
Owner 广州基赛生物科技有限公司
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