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Method and application of quickly screening arsenic ion aptamers

A nucleic acid aptamer, arsenic ion technology, which is applied in the analysis by chemical reaction of the material, the material analysis by observing the influence on the chemical indicator, the measurement of color/spectral characteristics, etc. The technology is time-consuming and other problems, to achieve the effect of simple operation, conducive to immobilization applications, and avoiding the limitation of PCR amplification primers

Inactive Publication Date: 2017-09-08
UNIV OF SHANGHAI FOR SCI & TECH
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  • Claims
  • Application Information

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Problems solved by technology

[0004] In order to solve the above problems, the present invention aims to provide a simple and fast aptamer screening method, which can quickly optimize the nucleic acid aptamer of arsenic ions, so as to overcome the time-consuming and high technical requirements of the existing aptamer screening technology. insufficient

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  • Method and application of quickly screening arsenic ion aptamers
  • Method and application of quickly screening arsenic ion aptamers
  • Method and application of quickly screening arsenic ion aptamers

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Embodiment

[0036] The nucleic acid sequences used in this example were chemically synthesized using the known classic phosphoramidite triester method. Its sequence is as follows:

[0037] As-40: TTA CAG AAC AAC CAA CGT CGC TCC GGG TAC TTC TTC ATC G,

[0038] As-40-2: TTA CAG AAC AAC CAA CCC GGG TAC TTC TTC ATC G,

[0039] As-40-4: TAG GGA GAT ACC AGC,

[0040] As-40-6: TTA CAG AAC AAC CGG TAC TTC TTC ATC G.

[0041] 1. Preparation of standard substance solution

[0042] The sodium chloride of taking different quality is made into the sodium chloride solution (0,0.1,0.3,0.5,0.7,0.9mol / L) of different concentrations with double distilled water; The DNA nucleic acid sequence (As-40, As- 40-2, As-40-4, As-40-6) were diluted with double distilled water to form gradient concentration aptamer solutions (0, 0.1, 0.3, 0.5, 1 μmol / L); weigh different masses of arsenous Sodium arsenite solution (0, 0.01, 0.05, 0.1, 0.5, 1 mmol / L) was prepared with double distilled water.

[0043] 2. Detection...

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Abstract

The invention relates to a reaction system of screening arsenic ion aptamers by means of a colorimetric method for optimizing an aptamer sequence of arsenic ions and detecting arsenic ions in water. The reaction system is characterized by comprising a nucleotide sequence, a nanogold solution and a sodium chloride solution, wherein the nucleotide sequence is TTA CAG AACAACCAACGTCGCTCCGGGTACTTCTTCATCG, TTA CAGAACAACCAACCCGGGTACTTCTTCATCG, TAG GGAGAT ACCAGC and TTACAGAACAACCGGTACTTCTTCATCG. The invention also provides an application of detecting arsenic ion concentration by means of the colorimetric method. As the screening process is simple and fast, an auxiliary reagent is relatively easily obtained, and measurement can be performed by means of naked eye judgment or a spectrophotometer, so that the screening difficulty of the aptamers is greatly reduced, and immobilized application and fast detection are facilitated.

Description

technical field [0001] The invention belongs to the field of environmental safety monitoring, and in particular relates to a method capable of rapidly screening arsenic ion nucleic acid aptamers and its application in the detection of arsenic ions. Background technique [0002] Arsenic is a toxic heavy metal that has a strong poisonous effect on animals and plants. It can continuously accumulate in soil and water, causing serious pollution. For the detection of heavy metals, a variety of detection methods have been developed, such as atomic absorption spectrometry, fluorescence method, electrochemical method, biosensor method and other methods. At present, there are chemical methods and instrument methods for the detection of arsenic. The chemical methods include the arsenic spot method and the silver salt method. Because the chemical method is cumbersome and the sensitivity is not high, it does not meet the needs of modern development. Although the instrument method has hig...

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Application Information

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IPC IPC(8): G01N21/78G01N21/31
CPCG01N21/31G01N21/78
Inventor 袁敏徐斐曹慧叶泰于劲松
Owner UNIV OF SHANGHAI FOR SCI & TECH
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