Method for preparing stichopus japonicus collagen emulsion
A collagen and emulsion technology, applied in the field of preparation of sashimi collagen emulsion, can solve problems such as insignificant skin care effect, and achieve the effects of eliminating skin pigmentation, maintaining softness and smoothness, and preventing arteriosclerosis.
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[0021] A preparation method for sashimi collagen emulsion, comprising the following steps:
[0022] Step 1: Weigh 50g of dried sea cucumber, soak in water at 60°C for 2 days, change the water 4-5 times a day, then continuously extract in 500mL of ethanol at 40°C for 2 days, replace with fresh ethanol every day, and finally dry at room temperature;
[0023] Step 2: Take the dried japonicus japonicus and react with superoxide dismutase for 4 hours. The ratio of superoxide dismutase to substrate is 1:4, pH 6.5, temperature 45°C. After the reaction is completed, boil for 5 minutes Inactivate the enzyme, and quickly cool to room temperature, centrifuge at 5000r.p.m for 30min, take the supernatant and add 100% ethanol to precipitate overnight, centrifuge at 5000r.p.m for 30min, rotate and volatilize at 40°C, and concentrate to obtain sashimi collagen;
[0024] Step 3: Weigh deionized water, heat it to 60°C, add carboxybenzyl group accounting for 1% of the mass percentage of deionize...
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