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GM2A genes as marker of male osteoporosis diagnosis and prognosis

An osteoporosis and GM2A technology, applied in the field of biomedicine, can solve the problems of high economic burden of osteoporosis patients, inability to detect whole body bones, and a small number of equipment.

Pending Publication Date: 2017-08-25
BEIJING FRIENDSHIP HOSPITAL CAPITAL MEDICAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is currently only used for the calcaneus, tibia and phalanges, and cannot detect the whole body bones
[0016] Dual-energy X-ray absorptiometry: It is the most ideal measurement method at present, and it is the gold standard for diagnosing osteoporosis. Expensive and therefore financially burdensome for diagnosis in osteoporosis patients

Method used

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  • GM2A genes as marker of male osteoporosis diagnosis and prognosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1 Screening of differentially expressed genes between male osteoporosis patients and normal males

[0047] 1. Research object:

[0048] Bone mineral density was detected, and 3 male patients with osteoporosis were selected, aged 60, 82, and 68 years old; 2 male patients were selected as the normal control group, aged 71 and 69 years old.

[0049] Inclusion criteria for patients with osteoporosis: (1) Those who meet the diagnostic criteria for osteoporosis, refer to the "Recommended Diagnostic Criteria for Osteoporosis in China (Second Draft); (2) All patients have given informed consent.

[0050] Exclusion criteria: elderly men with diabetes, severe liver and kidney diseases, thyroid diseases, and endocrine diseases; elderly men who have taken drugs for osteoporosis, such as sodium alendronate (except for intermittent small doses of calcium).

[0051] 2. Total RNA extraction from blood

[0052] (1) Pretreatment

[0053] Take fresh blood (peripheral blood) dir...

Embodiment 2

[0085] Example 2 QPCR experiment verification of differentially expressed genes between male osteoporosis patients and normal males

[0086] The GM2A gene screened out in Example 1 was selected for large sample verification.

[0087] 1. Research object:

[0088] Bone density was measured, and 30 male patients with osteoporosis, aged 60-82 years old were selected; 30 male patients with normal bone density, aged 60-82 years old, were selected as the control group.

[0089] Inclusion criteria for patients with osteoporosis: (1) Those who meet the diagnostic criteria for osteoporosis, refer to the "Recommended Diagnostic Criteria for Osteoporosis in China (Second Draft); (2) All patients have given informed consent.

[0090] Exclusion criteria: elderly men with diabetes, severe liver and kidney disease, thyroid disease, and endocrine disease; elderly men who have taken drugs for osteoporosis, such as sodium alendronate (except for intermittent small doses of calcium).

[0091] 2...

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Abstract

The invention discloses GM2A genes as a molecular marker for male osteoporosis diagnosis or prognosis. Through high throughput sequencing and QPCR studies, content of the GM2A genes in the blood of male osteoporosis patients is significantly less than that in normal males so that the GM2A genes can be used as a molecular marker for the male osteoporosis diagnosis or prognosis. A method of using the content of gene expression products in the blood to represent osteoporosis can be used for screening healthy people, and effectively prevent the osteoporosis.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to molecular markers for diagnosing male osteoporosis. Background technique [0002] Osteoporosis is a group of bone diseases caused by various reasons. The bone tissue has normal calcification, and the ratio of calcium salt to matrix is ​​normal. It is a metabolic bone lesion characterized by a decrease in the amount of bone tissue per unit volume. In most cases of osteoporosis, the loss of bone tissue is mainly due to increased bone resorption. Characterized by bone pain and susceptibility to fractures. [0003] The World Health Organization has listed osteoporosis as the second largest disease that endangers human health after cardiovascular disease. According to the latest "2013 China Osteoporotic Fracture Prevention Blue Book" released by the China Health Promotion Foundation, osteoporotic fracture is a kind of fragility fracture, that is, a fracture that can occur after minor trauma ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68G01N33/68
CPCC12Q1/6883C12Q2600/118C12Q2600/158G01N33/6893G01N2800/108G01N2800/52
Inventor 费琦
Owner BEIJING FRIENDSHIP HOSPITAL CAPITAL MEDICAL UNIV
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