A kind of ethanol freezing sterilization method and application of Cordyceps sinensis

A technology of Cordyceps sinensis and freeze sterilization, which is applied in the direction of medical preparations containing active ingredients, pharmaceutical formulas, sanitary equipment for toilets, etc. It can solve the problem that pollen color and composition are easy to change, it is difficult to achieve sterilization effect, and it is easy to oxidize Composition loss and other problems, achieve good sterilization effect, inhibit microbial growth, and solve the effect of enzymatic browning

Active Publication Date: 2019-11-19
劲牌持正堂药业有限公司
View PDF8 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantages of this method are: first, temperature control to -268.7°C is very demanding on equipment, although extreme low temperature and repeated freezing and thawing can cause mechanical damage and dehydration death of some microbial cells, but some microbial populations will be taken under low temperature conditions. The "self-hibernation" method, self-protection, and resurrection and reproduction when the conditions are right, it is difficult to achieve the expected sterilization effect
The Chinese patent publication number is CN 104415064A discloses the fresh-keeping storage technology of Cordyceps sinensis, cleans the fresh Cordyceps sinensis and carries out vacuum freeze-drying, then feeds ozone greater than 120ppm to sterilize for 60 minutes, the penetrating power of ozone sterilization method to material is limited, directly Sterilization of raw materials of Cordyceps sinensis will also cause a large loss of easily oxidized components
When microwave is used for sterilization, the sterilization effect is good, but the pollen color and composition are easy to change, which affects the quality; but when the pollen color and composition are well maintained, the sterilization effect is not ideal

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of ethanol freezing sterilization method and application of Cordyceps sinensis
  • A kind of ethanol freezing sterilization method and application of Cordyceps sinensis
  • A kind of ethanol freezing sterilization method and application of Cordyceps sinensis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The method for freezing and sterilizing Cordyceps sinensis in this embodiment includes the following steps:

[0022] (1) Ethanol infiltration: Crush the cordyceps to 60 mesh, add 70% ethanol solution to infiltrate the cordyceps powder for 5h, the volume of the ethanol solution is 5 times the weight of the cordyceps, and then add purified water to dilute the solution to the concentration of ethanol in the solution 35%;

[0023] (2) Pre-freezing: Pour the diluted solution into a freeze dryer at -65°C for 6 hours, and the thickness of the pan is ≤20 mm;

[0024] (3) Vacuum freeze-drying: After pre-freezing, perform sublimation drying at -45°C for 30h, with a vacuum of 100 µbar; then analyze and dry at 15°C for at least 2h with a vacuum of 10 µbar, and then the cordyceps can be sterilized and freeze-dried powder.

[0025] The sterilized lyophilized cordyceps powder obtained in this example and the cordyceps powder before sterilization were tested for hygiene, adenosine content, mo...

Embodiment 2

[0029] The method for freezing and sterilizing Cordyceps sinensis in this embodiment includes the following steps:

[0030] (1) Ethanol infiltration: Crush the cordyceps to 300 mesh, add 75% ethanol solution to infiltrate the cordyceps powder for 3h, the volume of the ethanol solution is 2.5 times the weight of the cordyceps, and then add purified water to dilute to the concentration of ethanol in the solution 15%;

[0031] (2) Pre-freezing: Pour the diluted solution into a freeze dryer at -60°C for 4 hours, and the thickness of the pan is ≤20 mm;

[0032] (3) Vacuum freeze-drying: After pre-freezing, carry out sublimation drying at -35°C for 22h, with a vacuum of 200 µbar; then analyze and dry at 18°C ​​for at least 2h with a vacuum of 50µbar to obtain the cordyceps sterilized freeze-dried powder .

[0033] The sterilized lyophilized cordyceps powder obtained in this example and the cordyceps powder before sterilization were tested for hygiene, adenosine content, moisture and other ...

Embodiment 3

[0037] The method for freezing and sterilizing Cordyceps sinensis in this embodiment includes the following steps:

[0038] (1) Ethanol infiltration: Crush the cordyceps to 1000 mesh, add 85% ethanol solution to infiltrate the cordyceps powder for 0.5h, the volume of the ethanol solution is 1 times the weight of the cordyceps, and then add purified water to dilute to the concentration of ethanol in the solution Is 5%;

[0039] (2) Pre-freezing: Pour the diluted solution into a freeze dryer at -50°C for 2 hours, and the thickness of the pan is ≤20 mm;

[0040] (3) Vacuum freeze-drying: After pre-freezing, perform sublimation drying at -20°C for 10 hours, with a vacuum of 400 µbar; then analyze and dry at 20°C for at least 2 hours with a vacuum of 100 µbar to obtain the Cordyceps powder.

[0041] The sterilized lyophilized cordyceps powder obtained in this example and the cordyceps powder before sterilization were tested for hygiene, adenosine content, moisture and other items. The res...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
thicknessaaaaaaaaaa
Login to view more

Abstract

The invention relates to an ethanol freezing sterilization method of caterpillar fungus and application thereof. The ethanol freezing sterilization method comprises the following steps of (1) soaking: crushing the caterpillar fungus into 60 to 1000 meshes; adding 70-85-percent ethanol for soaking treatment for 0.5 to 5h, wherein the volume of the ethanol solution is 1 to 5 times of the weight of the caterpillar fungus; then, adding pure water for dilution until the ethanol concentration is 5 to 35 percent; (2) pre-freezing: laying the diluted solution into a disc; putting the disc into an environment with the temperature being -65 to -50 DEG C for prefreezing for 2 to 6h; (3) vacuum freeze drying: performing sublimation drying for 10 to 30h at -45 to 20 DEG C and the vacuum degree of 100 to 400 mubar; performing desorption drying at 15 to 20 DEG C for at least 2h at the vacuum degree of 10 to 100 mubar; obtaining the caterpillar fungus sterilization freeze dry powder with the colony total quantity killing rate being higher than 99 percent. The ethanol freezing sterilization method has the advantages that the operation is simple; safety and non-toxicity are realized; the practical mass production requirements are met; good medicine, food and health care product development prospects are realized.

Description

Technical field [0001] The invention relates to the technical field of preparation of traditional Chinese medicine and health food, in particular to a method for freezing and sterilizing Cordyceps sinensis with ethanol and its application. Background technique [0002] Cordyceps sinensis is a fungus of the ergot family, which is a dry complex of the larvae and the corpse of the larvae of the Hepidiaceae. The nature and flavor are sweet and flat, enter the lung and kidney meridian, function to benefit the lung and kidney, relieve cough and asthma, invigorate deficiency, and nourish essence and qi. Used for chronic cough, wheezing, hemoptysis, impotence and nocturnal emission, waist and knee pain. "Preparation of Materia Medica": "Cordyceps sinensis, Gan Ping, protect lung and kidney, stop bleeding and resolve phlegm, relieve fatigue and cough". Zhao Xuemin’s "Supplements to Compendium of Materia Medica" records: "Cordyceps sinensis is mild in nature, replenishes the essence, and...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): A61L2/02A61L2/18A61L101/34A61K36/068A61K36/258A61K35/32A61K35/618A61K36/232A61K36/31
CPCA61K35/32A61K35/618A61K36/068A61K36/232A61K36/258A61K36/31A61L2/0011A61L2/0088A61L2202/21
Inventor 张雪元宋娟娟吴建辉周彬刘祖辉王晨李伟陆宇王忠稳赵凡
Owner 劲牌持正堂药业有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap