A kind of ethanol freezing sterilization method and application of Cordyceps sinensis

Abstract

The invention relates to an ethanol freezing sterilization method of caterpillar fungus and application thereof. The ethanol freezing sterilization method comprises the following steps of (1) soaking: crushing the caterpillar fungus into 60 to 1000 meshes; adding 70-85-percent ethanol for soaking treatment for 0.5 to 5h, wherein the volume of the ethanol solution is 1 to 5 times of the weight of the caterpillar fungus; then, adding pure water for dilution until the ethanol concentration is 5 to 35 percent; (2) pre-freezing: laying the diluted solution into a disc; putting the disc into an environment with the temperature being -65 to -50 DEG C for prefreezing for 2 to 6h; (3) vacuum freeze drying: performing sublimation drying for 10 to 30h at -45 to 20 DEG C and the vacuum degree of 100 to 400 mubar; performing desorption drying at 15 to 20 DEG C for at least 2h at the vacuum degree of 10 to 100 mubar; obtaining the caterpillar fungus sterilization freeze dry powder with the colony total quantity killing rate being higher than 99 percent. The ethanol freezing sterilization method has the advantages that the operation is simple; safety and non-toxicity are realized; the practical mass production requirements are met; good medicine, food and health care product development prospects are realized.

Description

Technical field [0001] The invention relates to the technical field of preparation of traditional Chinese medicine and health food, in particular to a method for freezing and sterilizing Cordyceps sinensis with ethanol and its application. Background technique [0002] Cordyceps sinensis is a fungus of the ergot family, which is a dry complex of the larvae and the corpse of the larvae of the Hepidiaceae. The nature and flavor are sweet and flat, enter the lung and kidney meridian, function to benefit the lung and kidney, relieve cough and asthma, invigorate deficiency, and nourish essence and qi. Used for chronic cough, wheezing, hemoptysis, impotence and nocturnal emission, waist and knee pain. "Preparation of Materia Medica": "Cordyceps sinensis, Gan Ping, protect lung and kidney, stop bleeding and resolve phlegm, relieve fatigue and cough". Zhao Xuemin’s "Supplements to Compendium of Materia Medica" records: "Cordyceps sinensis is mild in nature, replenishes the essence, and...

AI Technical Summary

Problems solved by technology

The disadvantages of this method are: first, temperature control to -268.7°C is very demanding on equipment, although extreme low temperature and repeated freezing and thawing can cause mechanical damage and dehydration death of some microbial cells, but some microbial populations will be taken under low temperature conditions. The "self-hibernation" method, self-protection, and resurrection and reproduction when the conditions are right, it is difficult to achieve the expected sterilization effect

The Chinese patent publication number is CN 104415064A discloses the fresh-keeping storage technology of Cordyceps sinensis, cleans the fresh Cordyceps sinensis and carries out vacuum freeze-drying, then feeds ozone greater than 120ppm to sterilize for 60 minutes, the penetrating power of ozone sterilization method to material is limited, directly Sterilization of raw materials of Cordyceps sinensis will also cause a large loss of easily oxidized components

When microwave is used for sterilization, the sterilization effect is good, but the pollen color and composition are easy to change, which affects the quality; but when the pollen color and composition are well maintained, the sterilization effect is not ideal

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