Kit for directly detecting folic acid medication sensitive gene by use of blood and application method thereof

Abstract

The inventiondiscloses a kit for directly detecting a folic acid medication sensitive gene by use of blood and an application method thereof. Human blood is adopted as a template, and a real-time fluorescence quantitative PCR system is formed by adding corresponding specific feature primers and fluorescent probes for detecting polymorphism of MTHFR C677T and MTHFR A1298C into the human blood respectively and amplifying a blood resistance taqpolymerase buffer and a blood resistance taqpolymerase. Through a Real time PCR reaction, a person skilled in the art determines the genotype of MTHFR according to the data collected by a fluorescence quantitative PCR instrument. The invention has the advantages of unnecessity of nucleic acid extraction, high speed and time saving, high sensitivity and easy judgment, low cost and the like, and is suitable for clinical detection and promotion.

Description

technical field [0001] The invention relates to the field of medical detection, and more specifically relates to a kit for directly detecting folic acid drug-sensitive genes in blood and a method for using the same. Background technique [0002] In recent years, with the development of biology, medicine, forensic science and other related fields, fluorescent PCR technology has been more and more widely used. As an important biological analysis sample, blood contains a large amount of DNA polymerase inhibitory substances, such as heme, miscellaneous protein, fat, etc. in blood. Therefore, based on the prior art, the fluorescent PCR method cannot directly amplify the nucleic acid in the blood. Usually, in order to complete the fluorescent PCR amplification of the nucleic acid of the blood sample, the nucleic acid substance must be purified from the blood first. It generally includes the following three processes: one is to rupture the cell membrane and nuclear membrane; the ...

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to provide a kit for direct detection of folic acid drug-sensitive genes in blood with high amplification efficiency and low cost, aiming at solving the problem of prior art when performing fluorescent quantitative PCR amplification on blood. Nucleic acid extraction and purification of blood, resulting in a large loss of nucleic acid, increased cross-contamination and the use of toxic reagents, and quickly obtained the SNP typing of MTHFR to guide folic acid medication

Images