Measurement method of porcine 2-cell embryo volume
A measurement method and cell technology, which is applied in the field of measuring the volume of pig 2-cell embryos, can solve the problem of inaccurate estimation of the volume of pig 2-cell embryos, and achieve the effect of improving production efficiency and developmental potential
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Embodiment 1
[0044] A method for measuring the volume of pig 2-cell embryos, comprising: pig 2-cells, the method comprising the steps of:
[0045] (1) Make a regression curve
[0046] A. Stain the 2-cell cleavage embryo, place the polar body at the 12 o'clock position of the embryo, take pictures under the microscope, use a rectangular frame to correct the edge of the measurement data and then measure the length, respectively get the left cell width LX1, the left cell length LY1, and the right cell length Cell width RX1, right cell length RY1;
[0047] B. Rotate 90 degrees along the direction of the cleavage groove, rotate the polar body to the top of the embryo, take the second photo, use a rectangular frame to correct the edge of the measurement data and then measure the length to obtain the left cell width LX2, the left cell length LY2, and the right cell Width RX2, right cell length RY2;
[0048] C. From the LX1, LX2, LY1, LY2, RX1, RX2, RY1, and RY2 measured in steps A and B, calcul...
Embodiment 2
[0055] According to the method for measuring the volume of pig 2-cell embryos described in Example 1, the embryo staining agent is Hoechst33342.
Embodiment 3
[0057] According to the method for measuring the volume of pig 2-cell embryos described in Example 1 or 2, in the steps A, B, and D, when taking pictures, focus on the pictures for five consecutive times to ensure that the maximum length is obtained.
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