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Application of Stevioside in Promoting the Growth of Chlorella

A technology of stevia and chlorella, which is applied to microorganism-based methods and uses added compounds to stimulate growth, microorganisms, etc., can solve the problems of slow growth and long growth cycle, and achieve the effect of improving culture efficiency and increasing quantity

Active Publication Date: 2020-07-31
上海利康精准医疗技术有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, in the cultivation of chlorella, there are problems of long growth cycle and slow growth. Stevioside has been used in high-power sweeteners, etc., and its function has many advantages. In the prior art, stevioside or stevia rebaudiana have not been used Study on the application of leaching solution to the cultivation of Chlorella

Method used

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  • Application of Stevioside in Promoting the Growth of Chlorella
  • Application of Stevioside in Promoting the Growth of Chlorella
  • Application of Stevioside in Promoting the Growth of Chlorella

Examples

Experimental program
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Effect test

Embodiment 1

[0028] Add 2mM (0.09g) stevioside to 50mL of algae fluid (culture fluid), and another control (without stevioside), culture at 25°C for 5 days under 4000Lux light (shake regularly every day), count with hemocytometer Compared with the blank control, the number of chlorella increased by 107%.

Embodiment 2

[0030] 4mM (0.14g) stevioside was added to 50mL of algae liquid (culture solution), and another control (without stevioside) was cultured at 25°C for 5 days under the condition of 4000 Lux light intensity (shaking regularly every day). The growth of chlorella was detected by hemocytometer and optical density measurement, and compared with the blank control, the number of chlorella increased by 203%.

Embodiment 3

[0032] 6mM (0.27g) stevioside was added to 50mL of algae liquid (culture liquid), and the other part was used as a control (without stevioside), cultured at 25°C and 4000 Lux light intensity for 5 days (shaking regularly every day), The growth of chlorella was detected by hemocytometer and optical density measurement, and compared with the blank control, the number of chlorella increased by 368%.

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Abstract

The invention discloses application of stevioside in promotion of growth of chlorella. With respect to the prior art, the invention provides application of stevioside or stevia rebaudiana leaching liquor in promotion of the growth of the chlorella and improvement of culture efficiency. Under certain conditions, a right amount of stevioside or stevia rebaudiana leaching liquor is added, the growth rate of the chlorella can be obviously increased, and the quantity of the chlorella in a culture solution is increased, so that the culture efficiency and yield of the chlorella are improved, and the stevioside or stevia rebaudiana leaching liquor is applicable to further popularization and application.

Description

technical field [0001] The invention discloses the application of stevioside or stevia extract in promoting the growth of chlorella, belonging to the new application field of stevioside. Background technique [0002] Chlorella (Chlorophyta) Chlorophyceae (Chlorophyceae) Chlorococcales (Chlorococcales) Oocystaceae (Oocystaceae) Chlorella (Chlorolla) general unicellular algae (Cao Yuntao, Kong Yingbao, He Yu Qin, Li Longnan, Xia Chungu. Effects of different nutritional methods on the growth characteristics and cell composition of Chlorella vulgaris[J]. Food and Fermentation Industry, 2011,37(10),45-51). It is rich in proteins, lipids, polysaccharides, dietary fiber, vitamins, trace elements and active metabolites. Its polysaccharides and glycoproteins have anti-tumor, anti-pathogen, anti-viral infection and immune-enhancing activities. It is becoming a popular food in recent years. Research hotspots (Kong Weibao, Li Longnan, Zhang Ji, Xia Chungu. The nutritional and health fu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/38C12N1/12C12R1/89
CPCC12N1/12C12N1/38
Inventor 陈育如尹慧慧郭欢欢杨州王小军蔡淮宇
Owner 上海利康精准医疗技术有限公司
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