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A method for efficient and rapid purification of bacterial cellulose

A technology of bacterial cellulose and cellulose, which is applied in the field of efficient and rapid removal of culture medium and bacterial cells in bacterial cellulose, can solve the problems of bacterial cellulose membrane impurity, odor, film color deepening, etc., to shorten the purification time, The effect of shortening time and cost

Active Publication Date: 2019-03-29
TIANJIN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Experiments have confirmed that high-temperature boiling of alkaline solution has limited ability to purify bacterial cellulose produced by fermentation, and it takes a long time. During the purification process, a large amount of peculiar smell will be produced, polluting the air
[0008] The pure strong alkali soaking method cannot completely remove the bacterial cells in the cellulose and the treatment cycle is long. Taking the bacterial fiber membrane with a diameter of 10±0.5 cm and a thickness of 0.8±0.05cm as an example, strong alkali can lyse the cells. It takes about 140h-160h to remove bacterial cells in bacterial cellulose by soaking in 0.1mol / L sodium hydroxide solution, but the purified bacterial cellulose membrane obtained is not pure
The boiling method cannot remove the bacterial cells and medium in the bacterial cellulose, and will cause the color of the film to deepen, and produce a lot of odor, causing air pollution

Method used

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  • A method for efficient and rapid purification of bacterial cellulose
  • A method for efficient and rapid purification of bacterial cellulose
  • A method for efficient and rapid purification of bacterial cellulose

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The method for efficiently and rapidly purifying bacterial cellulose comprises the following steps:

[0034] In the first step, the bacterial cellulose is taken out from the fermentation medium, and then washed twice with deionized water to remove the medium and bacteria on the surface of the bacterial cellulose, and the mass of the cellulose after washing is weighed as 60 g.

[0035] In the second step, the bacterial cellulose obtained in the first step is put into a beaker, and 300ml of 0.1mol / L sodium hydroxide solution is added to the beaker, and the beaker is put into an ultrasonic cleaner.

[0036] The third step is to turn on the ultrasonic cleaner, set the temperature parameter to 30°C, and the power parameter to 400W for ultrasonication, and replace the strong alkali solution of sodium hydroxide 3 times. Until the cellulose is all milky white.

[0037] In the fourth step, the product obtained in the third step was treated with 0.1 mol / L acetic acid solution, sti...

Embodiment 2

[0040] The method for efficiently and rapidly purifying bacterial cellulose comprises the following steps:

[0041] In the first step, the bacterial cellulose is taken out from the fermentation medium, and then washed three times with deionized water to remove the medium and bacteria on the surface of the bacterial cellulose, and the mass of the cellulose after washing is weighed as 60 g.

[0042] In the second step, the bacterial cellulose obtained in the first step is put into a beaker, and 300ml of 0.15mol / L sodium hydroxide solution is added to the beaker, and the beaker is put into an ultrasonic cleaner.

[0043] The third step is to turn on the ultrasonic cleaner, set the temperature parameter to 50°C, and the power parameter to 500W for ultrasonic cleaning and purification, and replace the sodium hydroxide strong alkali solution 3 times during the cleaning process. until the cellulose is milky white.

[0044] In the fourth step, the product obtained in the third step w...

Embodiment 3

[0047] The method for efficiently and rapidly purifying bacterial cellulose comprises the following steps:

[0048] In the first step, the bacterial cellulose is taken out from the fermentation medium, and then washed three times with deionized water to remove the medium and bacteria on the surface of the bacterial cellulose, and the mass of the cellulose after washing is weighed as 60 g.

[0049] In the second step, the bacterial cellulose obtained in the first step is put into a beaker, and 350ml of 0.2mol / L sodium hydroxide solution is added to the beaker, and the beaker is put into an ultrasonic cleaner.

[0050] The third step is to turn on the ultrasonic cleaner, set the temperature parameter to 60°C, and the power parameter to 700W for ultrasonic cleaning and purification, and replace the sodium hydroxide strong alkali solution 3 times during the cleaning process. until the cellulose is milky white.

[0051] In the fourth step, the product obtained in the third step wa...

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Abstract

The invention belongs to the field of cellulose edulcoration after bacterial cellulose is produced by fermentation, and particularly relates to a method for efficiently and rapidly purifying bacterial cellulose. The method comprises the following steps: taking out bacterial cellulose from a fermentation medium, washing with deionized water, placing in a container, adding 0.05 to 0.25mol / L strong alkali solution to the container, performing ultrasonic cleaning purification at 25 to 85 DEG C, and 200w to 1500w, in the cleaning process, replacing the strong alkali solution for 2 to 5 times, until the bacteria cellulose is milky white, wherein the ratio of the volume of the strong alkali solution to the mass of the bacterial cellulose is not less than 3 to 1; treating the alkali-washed bacterial cellulose with an acid solution under magnetic stirring for 10 to 60 minutes and then washing with deionized water to neutral. Compared with the prior art, the time for achieving the same purification effect is shortened by 2 to 5 times.

Description

technical field [0001] The invention belongs to the field of cellulose purification after bacterial cellulose is produced by a fermentation method, and in particular relates to a method for efficiently and quickly removing culture medium and bacterial cells in bacterial cellulose. Background technique [0002] Bacterial cellulose (BC) is produced by the synthesis of microorganisms that can produce bacterial cellulose, and the bacterial cellulose raw material obtained by fermentation of microorganisms that can produce bacterial cellulose can be obtained after proper treatment. Biocellulose membranes with high hydrophilicity, high mechanical strength and extremely high biocompatibility (biocompatibility) make biocellulose membranes widely used in various fields, such as filtration membranes in the industrial field, dietary fiber in the food industry and Artificial skin, artificial blood vessels, etc. in the biomedical field. [0003] Bacterial cellulose has been discovered fo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B15/02
CPCC08B15/02
Inventor 钟成贾士儒李博侯颖刘淼刘伶普谭之磊韩培培吕和鑫
Owner TIANJIN UNIV OF SCI & TECH
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