Biosynthesis of 1,3-butanediol

Abstract

This document describes biochemical pathways for producing 1,3-butanediol using a polypetide having Beta-ketothiolase activity to form a 3-oxo-5-hydroxypentanoyl-CoA intermediate that can be enzymatically converted to 1,3-butanediol, as well as recombinant hosts producing 1,3-butanediol.

Description

[0001] field of invention [0002] The present invention provides a method for the biosynthesis of 3-oxo-5-hydroxypentanoyl CoA using a polypeptide having β-ketothiolase activity and using a CoA transferase, thioesterase, decarboxylase, or secondary alcohol One or more polypeptides with dehydrogenase activity, or a recombinant host cell expressing one or more such enzymes enzymatically converts 3-oxo-5-hydroxypentanoyl CoA to 1,3-butanediol ( 1,3-BDO; also known as 1,3-butene glycol). [0003] Background of the invention [0004] 1,3-BDO is used for various purposes as a starting unit for the production of various compounds such as fragrances, pheromones, pesticides, penems and carbapenems. 1,3-BDO can generally be produced chemically by (1) hydrogenation of 4-hydroxy-2-butanone using a ruthenium complex of phosphine-aminophosphine and (2) from threonine in the presence of bromide ions Acids undergo nitrous deamination followed by esterification and reduction. However, this ...

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Problems solved by technology

However, this method is usually energy-intensive, involves multiple steps, consumes large amounts of solvents, and / or produces large amounts of by-products, limiting large-scale production.

In particular, these methods are uneconomical for large-scale production of 1,3-butadiene via catalytic dehydration of 1,3-BDO

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