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Collection device for plant root exudates and collection method for xerophyte root exudates

A root exudate and collection device technology, applied in the direction of sampling devices, etc., can solve the problems of easy breeding of microorganisms, lack of mechanical resistance, blockage of collection devices, etc., to achieve the effect of improving accuracy and reliability, reducing time, and avoiding mixing

Active Publication Date: 2017-07-28
INST OF SOIL FERTILIZER & RESOURCE ENVIRONMENT JIANGXI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Therefore, the technical problem to be solved by the present invention is to overcome the defect that the collecting device in the prior art is prone to clogging, thereby providing a plant root exudate collecting device
[0005] Another technical problem to be solved by the present invention is to overcome the defects that microorganisms are easy to breed in the device for collecting root exudates in situ in soil culture in the prior art, and the roots are easy to rot and lack mechanical resistance in the hydroponic environment, thereby providing a dry Collection method of plant root exudates

Method used

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  • Collection device for plant root exudates and collection method for xerophyte root exudates
  • Collection device for plant root exudates and collection method for xerophyte root exudates
  • Collection device for plant root exudates and collection method for xerophyte root exudates

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Embodiment 1

[0046] This embodiment discloses a method for collecting xerophyte root exudates, comprising:

[0047] (1) The soil culture variety is Ganzhi No. 9, which is specifically potted sesame seedlings until it grows to the full flowering stage, and the potting soil selected for use is regular stubble soil;

[0048] (2) Take out the sesame seeds in full flowering stage, rinse the roots slowly with distilled water for 3 times, remove all the soil on the roots, submerge all the roots in the culture solution, ventilate the culture solution with an air pump, and cultivate in water for 5 days, The average daily temperature during hydroponics is 29°C, and the culture medium is replaced once a day. The replaced culture medium is stored in a refrigerator at a temperature of 4°C. The liquids were combined to obtain the total liquid;

[0049] Wherein, in the process of hydroponics, if the root system is exposed to the water surface, distilled water can be added to the culture medium to ensure...

Embodiment 2

[0055] This embodiment discloses a method for collecting xerophyte root exudates, comprising:

[0056] (1) The soil culture variety is Ganzhi No. 9, which is specifically potted sesame seedlings until it grows to the full flowering stage, and the potting soil selected is continuous cropping soil;

[0057] (2) Take out the sesame seeds in full flowering stage, rinse the roots slowly with distilled water for 3 times, remove all the soil on the roots, submerge all the roots in the culture solution, ventilate the culture solution with an air pump, and cultivate in water for 7 days. The daily average temperature during hydroponics is 29°C, and the culture medium is replaced once a day. The replaced culture medium is stored in a refrigerator at a temperature of 4°C. Merge to obtain the total liquid;

[0058] Wherein, in the process of hydroponics, if the root system is exposed to the water surface, distilled water can be added to the culture medium to ensure that the roots are alwa...

Embodiment 3

[0064] This embodiment discloses a method for collecting xerophyte root exudates, comprising:

[0065] (1) soil cultivation kind is golden jute, is specifically potted sesame seedlings until it grows to full flowering stage, and the potted soil of selection is regular stubble soil;

[0066] (2) Take out the sesame seeds in full flowering stage, slowly rinse the roots 3 times with distilled water, remove all the soil on the roots, submerge all the roots in the culture solution, ventilate the culture solution with an air pump, and cultivate in water for 6 days, The average daily temperature during hydroponic cultivation is 25°C, and the culture medium is replaced every 2 days. The replaced culture medium is stored in a refrigerator at a temperature of 4°C. All the culture fluids were combined to obtain the total fluid;

[0067] Wherein, in the process of hydroponics, if the root system is exposed to the water surface, distilled water can be added to the culture medium to ensure...

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Abstract

The invention provides a collection device for plant root exudates. A filter plate is arranged in a nutrient liquid barrel to effectively filter solid impurities in liquid to prevent the blockage of a connecting pipe, furthermore, an air blowing device is arranged, so that through holes on the filter plate can be dredged to prevent the through holes on the filter plate from being blocked. The invention further provides a collection method for xerophyte root exudates. A method of combining soil culture and water culture is adopted to reduce the hydroponic time so as to effectively avoid phenomena of rotted roots and the like caused by long-term soaking of plant roots in the liquid, the collection method is suitable for xerophytes, and makes the culture environment more adapted to the growth environment of the xerophytes after carrying out the water culture transiently to further improve the connection accuracy and reliability of the root exudates.

Description

technical field [0001] The invention relates to the technical field of collecting root exudates, in particular to a device for collecting plant root exudates and a method for collecting xerophyte root exudates. Background technique [0002] The root system of plants is not only an organ of absorption and metabolism, but also a powerful secretory organ. During the growth of plants, on the one hand, plant roots absorb nutrients and water from the growth medium for the aboveground growth, and on the other hand, secrete low-molecular-weight organic compounds (sugars, Amino acids, phenolic acids and other secondary metabolites), high molecular weight viscose (polysaccharides, proteins, some phospholipids), and cell exfoliation and lysates, namely root exudates. Root exudates can not only change the physical and chemical properties of soil, the state of mineral elements in soil, the distribution of soil biological communities, and soil enzyme activity, but also affect the growth ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/20
CPCG01N1/20Y02P60/21
Inventor 吕丰娟张文猛魏林根肖运萍汪瑞清徐磊陈静蕊袁展汽林洪鑫李瑶
Owner INST OF SOIL FERTILIZER & RESOURCE ENVIRONMENT JIANGXI ACAD OF AGRI SCI
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