Culture method of phaeocystis globosa

Abstract

The invention discloses a culture method of phaeocystis globosa, and relates to red-tide algae. The culture method comprises the following steps: inoculating an algae solution of non-sterile phaeocystis globose cultured to a log phase into a sterile f / 2 culture medium, inoculating algae seeds into an f / 2-containing triangular flask for culturing the algae seeds; inoculating all the algae solution into the triangular flask containing sterile f / 2 culture medium for culturing to the log phase; inoculating all the algae solution into a sterilized algae culturing cylinder containing treated seawater, adding nutrient salt: 10 mL of a first solution, 5 mL of a second solution and 1 mL of a third solution; culturing under an illumination period of 12 h / 12 h and a ventilation volume of 9 L / min, monitoring a fluorescence value in the morning and the evening, wherein a fluorescence intensity of 100 corresponds to 1000 cells / mu L; after growing one day, adding 6 L of the treated seawater and an equivalent amount of nutrient salt to the algae culturing cylinder; monitoring the fluorescence value at a wavelength of 440 / 680 nm once in the morning and the evening; adding 20 mL of the first solution to the algae culturing cylinder; and monitoring the fluorescence value in the morning and the evening until growing to a cell concentration.

Description

technical field [0001] The invention relates to a red tide alga—Phaeocystis sphaeroides, in particular to a method for cultivating Phaeocystis sphaeroides stably cultivated in a 20L large-volume environment. Background technique [0002] Red tide, also known as harmful algal bloom, refers to the phenomenon that in a certain sea area, planktonic microalgae, protozoa or bacteria in the ocean multiply or gather in large numbers under suitable environmental conditions, causing water quality to deteriorate and sea water to change color. Marine ecological anomalies [1]. As a kind of global water pollution, red tide not only causes serious harm to marine environment, marine fishery and mariculture, but also brings immeasurable losses to the world economy, and threatens the survival of human beings [2]. Only after we fully understand the causes of red tides, the harm of red tides to human beings, and the impact of red tides on the world economy can we better prevent the occurrence ...

AI Technical Summary

Problems solved by technology

[0010] The traditional method is only suitable for cultivating algae in a sterile, small-volume environment, and it is very difficult to cultivate in a 20L large-volume sterile environment

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