Preparation method of pla1 type n-3 polyunsaturated fatty acid phospholipid

Abstract

The invention provides a method for preparing PLA1 type n-3 polyunsaturated fatty acid phospholipids and PLA1 type n-3 polyunsaturated fatty acid phospholipids prepared by the method of the invention. Compared with the effect in the prior art that the catalytic reaction at the PLA1 position is not more than 66% achieved by using commercial enzymes such as Lecitase Ultra, TL IM, and SP435, the method of the present invention greatly improves the production of PLA1-type functional phospholipids. Rate.

Description

technical field [0001] The invention belongs to the field of using biocatalysis to prepare lipids, and specifically relates to the preparation of PLA1 type n- 3 methods of polyunsaturated fatty acid phospholipids. Background technique [0002] Polyunsaturated fatty acid (PUFA) refers to a class of fatty acids with not less than 18 carbon atoms and more than two double bonds; among them, the PUFA whose first double bond appears at the third position of the methyl end of the carbon chain is called n -3 polyunsaturated fatty acids. Typical examples of n-3 polyunsaturated fatty acids include docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA). A large number of pharmacological and animal experiments have proved that DHA is necessary for the normal development of the retina and the normal function of the nervous system. In the brain and retina, DHA accounts for about 20% and about 35% of the total fatty acids there, respectively, and exists mainly in the form of phospho...

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Problems solved by technology

However, it is well known that free substrates significantly reduce the lifetime of immobilized enzymes compared to ester substrates, thereby increasing processing costs

More importantly, since free fatty acids are not suitable for direct consumption by the human body, further purification steps are required in the above processes to remove a large amount of free fatty acids in the product, which again increases the production cost

[0021] In addition, the process of reference 9 involves the addition of ethylene glycol, which is not yet a food additive allowed by the state, so the industrial feasibility is poor

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