A molecular marker closely linked to kiwi fruit soluble solids trait qtl locus and its application

A kiwifruit, soluble technology, applied in the field of molecular biology and genetic breeding, can solve the problems of molecular marker research that has not yet been carried out, and achieve the effects of convenient and fast detection methods, accurate and fast screening, and saving production costs

Active Publication Date: 2018-06-12
WUHAN BOTANICAL GARDEN CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the QTL mapping of kiwifruit important quality traits soluble solids and the discovery of molecular markers related to soluble solids have not yet been carried out.

Method used

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  • A molecular marker closely linked to kiwi fruit soluble solids trait qtl locus and its application
  • A molecular marker closely linked to kiwi fruit soluble solids trait qtl locus and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Acquisition of molecular markers linked to main effect QTL and development of kiwifruit soluble solids:

[0023] ① In this example, the kiwifruit 'MT570001' with a small soluble solid content and the Chinese kiwifruit 'Guihai No. 4' with a large soluble solid content were used to construct F 1 Separate groups. In the 6th year of the group's results, 125 F 1 The individual plants of the offspring of the population were the research objects.

[0024] ②A total of 125 individual plant leaves were collected from the population, and the total DNA was extracted by CTAB method. According to the RADseq method, the libraries of Chinese kiwifruit 'Guihai 4', Shanli kiwifruit 'MT570001' and 125 offspring were constructed and sequenced.

[0025] ③ Filter the sequencing data to remove sequences with low-quality base content greater than 50%, and remove sequences with sequencing adapter contamination and a large number of repetitions. Use the software SOAP2 to compare the sequenci...

Embodiment 2

[0035] The application of a molecular marker closely linked to the kiwifruit soluble solids trait QTL site in kiwifruit breeding, the steps are:

[0036] (1) F 1 Generation, choose F 1 60 individual plants in the generation population were the research objects.

[0037] (2) At the ripening stage of the fruit, collect F 1 The fruit of 60 individual plants in the generation group, 20 fruits were randomly collected for each sample, and the soluble solids content was measured using an ATAGO Palette PR-32α digital refractometer.

[0038] (3) Extract F 1 Generation of total DNA of 60 individual plants, according to Premix Ex Taq TM II (Tli RNaseHPlus), ROX plus kit instruction, design 20uL reaction system: 5ng·uL -1 Kiwifruit genomic DNA template 2ul, 2×SYBR Premix 10ul, 50×ROX Dye II 0.4ul, 10uM SSC-F and SSC-R 0.8ul each, double distilled water to make up the rest. The amplification program was: pre-denaturation at 95°C for 5 s, annealing at 60°C for 1 min; denaturation at...

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Abstract

The invention discloses a molecular marker closely linked with a soluble solid trait QTL site of a kiwifruit fruit and application of the molecular marker. The primers designed according to the molecular marker are SSC-F:5'-TTGTCTTGATAGGCCCCTCG-3' and SSC-R:5'-CAACAAGACATGGTTCCTCACA-3'. The molecular marker firstly locates a main-effect QTL site for controlling soluble solid in a kiwifruit, can explain phenotypic variance of 19.61%; and the molecular marker associated with the soluble solid trait is detected, so that the soluble solid of the kiwifruit fruit can be predicated, and therefore, an excellent individual plant with relatively high soluble solid weight can be accurately and quickly screened out.

Description

technical field [0001] The invention belongs to the technical field of molecular biology and genetic breeding, and more specifically relates to a molecular marker closely linked to a QTL site of kiwifruit soluble solids traits and its application. Background technique [0002] Kiwi fruit is rich in vitamin C, dietary fiber and various mineral nutrients, and is a healthy fruit favored by consumers. China is the original origin center of the genus Actinidia. Actinidia is extremely rich in resources and has high genetic diversity. China has about 96% of the world's total resources of Actinidia species. Relying on abundant natural resources, breeding new varieties with excellent traits plays a key role in the healthy and stable development of the kiwifruit industry. Soluble solids are the general term for all compounds dissolved in water, including sugars, acids, vitamins, minerals, etc., and are one of the important factors affecting the quality and flavor of fruits. Therefor...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 黄宏文钟彩虹张琼张鹏李大卫
Owner WUHAN BOTANICAL GARDEN CHINESE ACAD OF SCI
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