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Dyeing method for conveniently observing digestive systems of predatory mites and prey tetranychidae of predatory mites

A technology of digestive system and dyeing method, which is applied in animal husbandry and other fields, can solve the problems of not observing the digestive system of predator mites and their prey spider mites, and achieve the effect of simple operation

Inactive Publication Date: 2017-06-20
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, so far, there is no relevant method to observe the digestive system of predator mites and their prey spider mites in China.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0008] Example Dyeing method of prey spider mites and predatory mites

[0009] Pick several spider mites into a 2ml centrifuge tube and starve for 24 hours for later use. Then insert 3-4 branches of fresh kidney bean branches with 3-4 leaves in each conical flask filled with 500 ml of 0.1% sodium fluorescein aqueous solution. After 24 hours, the leaves of kidney beans can be completely dyed. The leaves are connected with spider mites, and after 24 hours, the fluorescence of the whole body of the spider mites can be observed under a fluorescent stereomicroscope at a higher magnification. After the spider mites are stained with fluorescent light, feed the predatory mites with starved predatory mites on the leaves for 24 hours, or pick the dyed spider mites from the leaves and feed them with a single head of the hungry predatory mites in the breeding chamber. Under a higher magnification of the microscope, it can be observed that the digestive tract of the predatory mite is stai...

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Abstract

The invention relates to a dyeing method for conveniently observing digestive systems of predatory mites and prey tetranychidae of the predatory mites. The dyeing method is characterized by including the steps that 1-5 pieces of fresh kidney bean branches with two or above leaves are taken and inserted into a conical flask containing a 0.05% to 0.5%sodium-fluorescein water solution, tetranychidae hungering for 0 hour to 48 hours are placed on the leaves after 24 hours for feeding the tetranychidae, the digestive-system-conveniently-observed tetranychidae can be obtained after 12 hours to 48 hours, the tetranychidae continues to be used for feeding the predatory mites hungering for 0 hour to 48 hours, and the digestive-system-conveniently-observed predatory mites can be obtained after 12 hours to 48 hours. The dyeing method is easy to operate, and references can be provided for the aim that digestive tracts and whole digestive systems of the predatory mites and predatory-mite prey tetranychidae can be observed under a common phosphor-type microscope.

Description

technical field [0001] The invention relates to a dyeing method for conveniently observing the digestive system of predatory mites and their prey spider mites, belonging to the field of biological control of modern agricultural pests. Background technique [0002] At present, domestic research on mites is mainly limited to classification, breeding and application. There is little research on the microstructure of mites, especially the mechanism of the digestive system. [0003] The mite digestive system is an important structure for the study of mites eating and digesting food. It is of great significance to observe the direction of food in the mite body after the mite eats the food. . [0004] However, so far, there is no relevant method in China on how to observe the digestive system of predator mites and their prey spider mites. Contents of the invention [0005] The purpose of the present invention is to provide a dyeing method that is convenient for observing the di...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033
CPCA01K67/033
Inventor 徐学农王恩东吕佳乐贾冰红刘珊岳瑾董杰乔岩周长青张正伟王伟青庞博张乐
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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