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Method for predicting recurrence and metastasis time and prognosis of triple-negative breast cancer (TNBC) by establishing miRNAs (micro Ribonucleic Acids) expression level

A triple-negative breast cancer, recurrence and metastasis technology, applied in the field of bioengineering, can solve the problems of high recurrence rate and metastasis rate, easy drug resistance, poor prognosis, etc., and achieve the effect of improving prognosis and improving treatment effect.

Inactive Publication Date: 2017-06-13
何东宁
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Problems solved by technology

The postoperative recurrence and metastasis rates of TNBC are relatively high, drug resistance is easy, and the prognosis is poor. These are the key points and difficulties faced by TNBC treatment at present.
However, there is no report on the use of miRNAs to accurately predict the time and prognosis of TNBC recurrence and metastasis.

Method used

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  • Method for predicting recurrence and metastasis time and prognosis of triple-negative breast cancer (TNBC) by establishing miRNAs (micro Ribonucleic Acids) expression level
  • Method for predicting recurrence and metastasis time and prognosis of triple-negative breast cancer (TNBC) by establishing miRNAs (micro Ribonucleic Acids) expression level

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Embodiment Construction

[0022] A method for predicting the recurrence and metastasis time and prognosis of triple-negative breast cancer by establishing miRNAs expression levels, comprising the steps of:

[0023] 1. Collection of samples:

[0024] Normal breast tissue adjacent to the tumor was collected from patients with triple-negative breast cancer and some patients with breast fibroma. All cases were female and were finally diagnosed as invasive ductal carcinoma of the breast or simple hyperplasia of the breast. During the operation, breast specimens of clinically relevant size were taken, placed in cryopreservation tubes, quickly frozen in liquid nitrogen, and then transferred to a -80°C refrigerator for storage. Form treatment group cells and control group cells.

[0025] 2. RNA extraction and quantitative PCR;

[0026] 3. Poly A tailing of RNA samples:

[0027] PolyA tailing was performed on the RNA sample. The reaction system was: Total RNA 2|_ig, 10xPolyAPolymeraseBuffer 210x ATP Solutio...

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Abstract

The invention relates to a method for predicting recurrence and metastasis time and prognosis of a triple-negative breast cancer (TNBC) by establishing a miRNAs (micro Ribonucleic Acids) expression level. The method comprises the following steps: 1, sample collection; 2, RNA extraction and quantitative PCR (Polymerase Chain Reaction); S3, RNA sample Poly A tailing; S4, reverse transcription and quantitative PCR; S5, data analysis. As proved by the method disclosed by the invention, the expression levels of miR-4262, miR-670-3p, miR-152-3p and miR-216a-5p are remarkably relevant to disease-free survival time of 3 years and 5 years and total survival time, the recurrence and metastasis time of TNBC patients with high expression levels is often within 2 to 3 years, and the prognosis is bad. Specific treatment is performed on the part of patients, so that the recurrence and metastasis risk is lowered, and the prognosis is improved.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a method for predicting the recurrence and metastasis time and prognosis of triple-negative breast cancer by establishing the expression level of miRNAs. Background technique [0002] Triple-negative breast cancer (TNBC) is a highly malignant and aggressive type of breast cancer that lacks targets for endocrine therapy and targeted therapy, so chemotherapy is the main drug treatment besides surgery . The postoperative recurrence rate and metastasis rate of TNBC are relatively high, drug resistance is easy, and the prognosis is poor. These are the key points and difficulties faced by TNBC treatment at present. Therefore, accurately predicting the time of TNBC recurrence and metastasis and taking targeted measures such as chemotherapy are of positive value in improving the prognosis of TNBC patients and efficiently allocating medical resources. [0003] MicroRN...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6886C12Q2600/118C12Q2600/158C12Q2600/178
Inventor 何东宁马萍哈敏文
Owner 何东宁
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