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Bifidobacterium bifidum and its use

A technology of bifidobacteria bifidum and bacterial agent is applied in the field of microorganisms to achieve the effects of improving the water content of feces, wide application prospects and good tolerance.

Active Publication Date: 2020-08-25
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no monobacteria that relieves constipation, or bifidobacteria that mediate intestinal motility and other properties

Method used

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  • Bifidobacterium bifidum and its use
  • Bifidobacterium bifidum and its use
  • Bifidobacterium bifidum and its use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] Example 1: Tolerance dose experiment of mice when Bifidobacterium bifidum CGMCC NO.13632 was fed to mice

[0068] Resuspend the freeze-dried bacteria powder of Bifidobacterium bifidum CGMCC NO.13632 in skim milk powder to make a concentration of 4.0×10 9 CFU / mL bacterial suspension. Take 10 healthy male BALB / c mice with a body weight of about 25 g, give the suspension of this concentration orally once a day, observe for a week, and record the death and body weight.

[0069] The results of these tests are listed in Table 1. These results indicated that feeding concentrations of 4.0×10 9 CFU / mL of Bifidobacterium bifidum CGMCC NO.13632 had no significant impact on mice, no significant change in body weight, and no death. The appearance of the mice had no obvious pathological symptoms.

[0070] Table 1 Changes in body weight and death of mice

[0071]

[0072]

[0073] Note: -: no death of mice

Embodiment 2

[0074] Example 2: Relief effect of Bifidobacterium bifidum CGMCC NO.13632 on constipation in mice

[0075] Forty healthy male BALB / c mice weighing 20-25 g were randomly divided into 5 groups: blank control group, constipation model control group, Bifidobacterium bifidum CGMCC NO.13632 intervention group, Lactobacillus plantarum control group and phenolphthalein control group The treatment control group contained 8 mice in each group.

[0076] 14 days before the experiment, the mice in the intervention group were fed with the concentration prepared in Example 1 of this manual every day, which was 4.0×10 9 CFU / mL of Bifidobacterium bifidum CGMCC NO.13632 skim milk suspension 0.25mL, the Lactobacillus plantarum group was fed with the same amount of L.plantarumST-III, and the other three groups were fed with the same amount of bacteria-free skim milk. On the 15th-17th day of the test, the negative control group was given 0.25mL normal saline, and the other four groups were given ...

Embodiment 3

[0081] Example 3: Regulatory effect of Bifidobacterium bifidum CGMCC NO.13632 on constipation-related gastrointestinal peptides in serum of constipated mice

[0082]Forty healthy female BALB / c mice weighing 20-25 g were randomly divided into 5 groups: blank control group, constipation model control group, Bifidobacterium bifidum CGMCC NO.13632 intervention group, Lactobacillus plantarum control group and phenolphthalein control group The treatment control group contained 8 mice in each group. 14 days before the experiment, the mice in the intervention group were fed with the concentration prepared in Example 1 of this manual every day, which was 4.0×10 9 CFU / mL Bifidobacterium bifidum CGMCC NO.13632 skimmed milk suspension 0.25mL, Lactobacillus plantarum control group fed with the same amount of L.plantarumST-III, and the other 3 groups were fed with the same amount of bacteria-free skim milk . On the 15th-17th day of the test, the blank control group was given 0.25mL of nor...

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Abstract

The invention discloses a bifidobacterium bifidum capable of significantly alleviating constipation and an application thereof, belonging to the technical field of microbes. Bifidobacterium bifidum CGMCC NO.13632 provided by the present invention can well tolerate simulated gastrointestinal fluid, can well adhere to colon cancer cell HT‑29, and can significantly improve the feces water content and Shorten the time of the first black stool; in terms of improving the propulsion rate of the small intestine, Bifidobacterium bifidum CGMCC NO.13632 of the present invention is superior to the laxative phenolphthalein, showing the best effect, while significantly improving the pathological indicators of constipation, it also regulates the The secretion of constipation-related gastrointestinal regulatory peptides in serum does not have the toxic and side effects of drugs for treating constipation, so it is the first choice for the prevention and treatment of constipation. The bifidobacterium bifidum CGMCC NO.13632 is used to prepare a medicinal composition and fermented food for relieving constipation, and has very broad application prospects.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a bifidobacterium bifidum capable of significantly alleviating constipation and its application. Background technique [0002] Bifidobacterium is an essential source of nutrition for the human body. It can synthesize a variety of digestive enzymes and B vitamins, such as vitamin B1, B2, B6, B12, folic acid, niacin and other vitamins, to help the body digest some that cannot be digested by its own enzyme system Nutrients to promote the body's absorption of nutrients, while bifidobacteria can also synthesize, absorb and utilize a variety of B vitamins, and can use ammonia as a nitrogen source to synthesize amino acids and urea in the intestinal tract, reducing blood ammonia concentration, Improve the accumulation of protein in the body. In addition, bifidobacteria produce organic acids through metabolism to reduce the pH and Eh (oxidation-reduction potential) of the intesti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A61K35/745A61P1/10A23L33/135C12R1/01
CPCA23V2002/00A61K35/745C12N1/20C12N1/205C12R2001/01A23V2200/32
Inventor 陈卫王刚王琳琳田丰伟张秋香刘小鸣范大明赵国忠张白曦翟齐啸毛丙永郭敏杨波陆文伟赵建新张灏
Owner JIANGNAN UNIV
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